南方医科大学学报2016，Vol.36Issue(5)：645-648,4.
SUN5特异性抗体的制备及其应用
Preparation of SUN5-specific polyclonal antibody for detection of SUN5 expression in human germ cells
摘要
Abstract
Objective To prepare a specific polyclonal antibody against full-length SUN5 for detecting the expression of SUN5 in human germ cells. Methods Bioinformatic methods were used to compare the full-length SUN5 and its variant SUN5β, and a short peptide was designed based on the differential region to prepare SUN5 antibody. The prepared antibody was used to detect the expression of SUN5 in Ntera-2 cells and in human germ cells by Western blotting and immunofluorescence assay. Results The short peptide was correctly synthesized and SUN5 antibody was obtained and purified. Western blotting showed that the prepared antibody was capable of recognizing full-length SUN5 in Ntera-2 cells, and SUN5 expression was localized on the nuclear membrane and in the cytoplasm as shown by immunofluorescence assay. Using this antibody, we detected SUN5 expression in the spermatocytes, round spermatids and sperms in human germ cells. Conclusion We successfully prepared SUN5-specific antibody. SUN5 is expressed in the spermatocytes, round spermatids and sperms in human germ cells, suggesting its important role in spermatogenesis.关键词
SUN5/抗体/Western blot/免疫荧光/生殖细胞Key words
SUN5/antibody/Western blotting/immunofluorescence/germ cells引用本文复制引用
王静芳,黄利华,伍勇,杨林飞,任瑶,邢晓为..SUN5特异性抗体的制备及其应用[J].南方医科大学学报,2016,36(5):645-648,4.基金项目
国家自然科学基金（81170615） Supported by National Natural Science Foundation of China (81170615) （81170615）
