南方医科大学学报2016，Vol.36Issue(5)：649-654,6.
蛋白4.1家族在小鼠黑色素瘤细胞中的表达及其对细胞增殖的影响
Expression of protein 4.1 family in melanoma cell lines and its effect on cell proliferation
摘要
Abstract
Objective To detect the expression of protein 4.1 family members in mouse melanoma cell lines and evaluate their effect on cell proliferation. Methods PCR and Western blot were used to detected to the expression of protein 4.1 family members (4.1R, 4.1B, 4.1G, and 4.1N) at the mRNA and protein levels in B16 and B16-F10 cell lines. The expression plasmid vector pEGFP-N1-EPB41L3 carrying 4.1B gene sequence amplified from genomic RNA of mouse embryo fibroblasts was constructed and transiently transfected into mouse melanoma cells. The change in cell proliferation was assessed using MTT assay. Results The mRNA and protein expressions of all the protein 4.1 family members, with the exception of 4.1B, were detected in both B16 and B16-F10 cells. Transfection of cells with the eukaryotic expression vector pEGFP-N1-EPB41L3 markedly inhibited cell proliferation as compared with the non-transfected cells. Conclusion The eukaryotic expression vector carrying EPB41L3 sequence is capable of inhibiting the proliferation of mouse melanoma B16 and B16-F10 cells.关键词
黑色素瘤细胞/蛋白4.1/真核表达/抑制增殖Key words
mouse melanoma cells/protein 4.1/eukaryotic expression vector/inhibiting proliferation引用本文复制引用
门颖丽,康巧珍,丁聪,刘诗梦,江慧,王晓东,汲振余,刘鑫,王婷..蛋白4.1家族在小鼠黑色素瘤细胞中的表达及其对细胞增殖的影响[J].南方医科大学学报,2016,36(5):649-654,6.基金项目
国家自然科学基金（81373119，81571526） Supported by National Natural Science Foundation of China (81373119,81571526) （81373119,81571526）
