福建农业学报2016，Vol.31Issue(3)：217-224,8.DOI:10.19303/j.issn.1008-0384.2016.03.002
花生谷氨酸脱氢酶基因 AhGDH1的克隆与生物信息学分析
Cloning and Bioinformatic Analysis of AhGDH1 Gene from Arachis hy pogaea L
摘要
Abstract
A glutamate dehydrogenase gene from A rachis hypogaea L .was obtained by using the in silico cloning technique .Its sequence was amplified using primers designed according to the cDNA sequence .The sequencing confirmed and named the gene AhGDH1 with a GenBank accession number of KT933119. Through the bioinformatic analysis ,the composition of amino acid sequence ,basic physical and chemical properties ,subcellular localization , transmembrane region , signal peptide , guide peptide , and secondary and tertiary structures of the protein were studied .The results showed that the length of cDNA of the gene was 1 713 bp ,which included a 1 236 bp open reading frame and encoded 411 amino acids residue .The encoding protein of the AhGDH1 gene consisted of two typical conserved domains .The homology comparison and phylogenetic analysis indicated that the amino acid encoded by AhGDH1 gene in A .hypogaeawas highly homologous with those encoded by GDH1 gene in Cicer arietinum and others . Thus , it was determined that the protein was a glutamate dehydrogenase . The information provided for the expression analysis and function identification of GDH1 gene in A .hypogaea in the future .关键词
花生/谷氨酸脱氢酶基因/基因克隆/生物信息学分析Key words
peanut (A rachis hy pogaea L .)/glutamate dehydrogenase gene/gene cloning/bioinformatic analysis分类
农业科技引用本文复制引用
陈湘瑜,徐日荣,林栩松,唐兆秀..花生谷氨酸脱氢酶基因 AhGDH1的克隆与生物信息学分析[J].福建农业学报,2016,31(3):217-224,8.基金项目
福建省科技计划项目---省属公益类科研院所基本科研专项（2015R1026-10）；福建省农业科学院科技创新团队建设项目（CXTD －1-1301）；福建省农业厅科技项目 （）
