福建农业学报2016，Vol.31Issue(3)：230-235,6.DOI:10.19303/j.issn.1008-0384.2016.03.004
‘紫鹃’茶树紫叶和绿叶差异表达蛋白分析
Differentially Expressed Proteins in Green and Purple Leaves of Zijuan Tea
摘要
Abstract
The mechanism regulating the anthocyanin metabolism in the reddish violet‐shoot tea ,Zijuan (Camellia sinensis var .kitamura) ,was investigated .The isobaric tag for the relative and absolute quantification (iTRAQ ) method was applied to compare the differentially expressed proteins in the purple and green leaves of the tea plant . A total of 798 differentially expressed proteins were identified from them .In the purple leaves ,533 of the proteins were up‐regulated and 265 down‐regulated . They were categorized by their association with photosynthesis (accounting for 14.7% ) ,carbohydrate metabolism (5.1% ) ,transcriptional regulation (18.5% ) ,protein synthesis (11.4% ) , structural proteins (6.9% ) , signaling transduction (6.9% ) , amino acid metabolism (3.8% ) , anthocyanin metabolism (3.6% ) ,antioxidant stress (3.5% ) ,lipid metabolism (3.5% ) ,respiration ,or others .It appeared that the decrease in total soluble sugars in the purple leaves of Zijuan might relate to anthocyanin accumulation .关键词
‘紫鹃’茶树/iTRAQ/差异蛋白/花青素Key words
Camellia sinensis var .kitamura/iTRAQ/differentially expressed proteins/anthocyanin分类
农业科技引用本文复制引用
陈伟,吕晓杰,宋晓敏,邱智敏,梁猛,陈世品..‘紫鹃’茶树紫叶和绿叶差异表达蛋白分析[J].福建农业学报,2016,31(3):230-235,6.基金项目
福建省自然科学基金（2014J01079）；高等学校博士学科点专项科研基金 （）
