福建农业学报2016，Vol.31Issue(3)：236-241,6.DOI:10.19303/j.issn.1008-0384.2016.03.005
4种栽培模式下铁皮石斛提取物抑菌活性
Antimicrobial Activity of Extracts from Dendrobium o f f icinale Kimura et Migo Grown Under Four Cultivation Methods
摘要
Abstract
Four different methods ,i .e .,(A) bed planting under trees in forest ,(B) intergrowth under trees in forest ,(C ) suspending on trees in forest , and (D ) bed planting in greenhouse , were applied to cultivate Dendrobium of ficinale Kimura et Migo to compare the antimicrobial activity of the extracts from the plants .The results showed that the antimicrobial effect of the extract from the plants grown under (B) ,as indicated by the diameter of the inhibition zones (IZ) on petri dish medium ,was most significant on Staphylococcus aureus (12.64 mm dia .) ,Escherichia coli (11.93 mm dia .) ,and Serratia marcescens H30 (8.49 mm dia .) with the minimum inhibitory concentrations (MICs) of 15.62 ,31.25 ,and 250 g·mL -1 ,respectively .The extract from the plants grown under (A) had the best antimicrobial effects shown on Pseudomonas aeruginosa (IZ of 9.35 mm in dia .) and S .marcescens MG1 (IZ of 9.33 mm in dia .) with MICs of 62.5 and 125 g·mL -1 ,respectively .Among the 4 cultivation methods ,(B) yielded the highest extraction rates on polysaccharides (78.54% ) and ethanol soluble (36.01% ) from the plants . A regression analysis on the antimicrobial activities and chemicals of the extracts indicated a positive correlation between the polysaccharide content and the inhibition on S .A ureus and E .coli with correlation coefficients of 0.995 and 0.951 ,respectively ,(P<0.05 or P<0.01);while the ethanol soluble and the inhibition on S .marcescens correlated significantly with a correlation coefficient of 0.984 at P<0.05. Therefore ,it appeared that cultivation method affected the functional components in D .officinale ,and in turn ,the antimicrobial activity of the plant extracts .关键词
铁皮石斛/提取物/仿野生栽培/大棚床栽/抑菌活性Key words
Dendrobium officinale Kimuraet Migo/extract/bionic cultivation/greenhouse cultivation/bacteriostatic effect分类
农业科技引用本文复制引用
王珂珂,陈淑钦,王威,谢倩,陈清西..4种栽培模式下铁皮石斛提取物抑菌活性[J].福建农业学报,2016,31(3):236-241,6.基金项目
福建省科技计划重大专项 （）
