法医学杂志2016，Vol.32Issue(2)：123-125,3.DOI:10.3969/j.issn.1004-5619.2016.02.011
不同前处理方法提取牙齿DNA的比较
Comparison of Different Pretreatment Methods for DNA Extraction from Teeth
摘要
Abstract
Objective To com pare the concentration of teeth DNA extracted by three different pretreatm ent m ethods and to explore a sim ple, econom ical and practical pretreatm ent m ethod w ith high concentration of extracted DNA from teeth. Methods A total num ber of 21 m olars w ere collected from 7 corpses. The pretreatm ent of 3 m olars from each individual w as random ly perform ed by tooth crum b m ethod, ball-m illing m ethod and liquid nitrogen m illing m ethod and 50 m g tooth crum b w as w eight and DNA w as extracted by A utoM ate ExpressTM forensic DNA extraction system . Subsequently, the concentration of DNA and corresponding STR genotyping of three m ethods w ere com pared. Results The DNA concentration extracted by tooth crum b m ethod, ball-m illing m ethod and liquid nitrogen m illing m ethod w as 0.055 6-1.989 1 ng/μL , 0.036 6-1.175 6 ng/μL and 0.037 8-1.249 0 ng/μL , respectively. The DNA concentration ob-tained by tooth crum b m ethod w as higher (P<0.05) and the success rate of STR genotyping w as high. Conclusion C om bined w ith A utoM ate ExpressTM forensic DNA extraction system , tooth crum b m ethod is an efficient and feasible m ethod to extract DNA from teeth, w hich can be applied in forensic practice.关键词
法医遗传学/DNA/牙/提取法/前处理Key words
forensic genetics/D N A/tooth/extraction/pretreatm ent分类
社会科学引用本文复制引用
骆继怀,孙红兵,杨鑫,张子龙,王涛..不同前处理方法提取牙齿DNA的比较[J].法医学杂志,2016,32(2):123-125,3.基金项目
国家自然科学基金资助项目（81302622）；甘肃省青年科技基金计划资助项目（1308RJYA059）；甘肃省自然科学基金资助项目（145RJZA038）；甘肃省公安厅技术研究计划项目 （）
