口腔疾病防治2016，Vol.24Issue(5)：273-279,7.DOI:10.12016/j.issn.2096-1456.2016.05.003
O-GlcNAc通过抑制Wnt信号通路影响上唇发育的实验研究
Experimental study of O-GlcNAc on the influence of the upper lip development through inhibition of Wnt signa-ling pathway
摘要
Abstract
Objective To explore the role of Wnt signaling pathway in the process of epithelial layer's disappearance when Oxydiethanoldiethylene GlcNAc influence development of upper lip. Methods The over/lower-expression of O-Gl-cNAc and reverse-high expression experimental group was constructed by transplanting beads into the upper lip of chicken embryo which contain O-GlcNAc promoter OGT ( O-GlcNAc transferase) , O-GlcNAc inhibitor OGA ( O-GlcNAcase) or OGA inhibitor STZ( streptozoticin) . and kept incubation for 24 h. Phenotypic analysis of skeletal and morphological chan-ges were performed by using Alizarin Red/Alcian Blue method. TUNEL assay was performed to analyze the cell apopto-sis. Immunohistochemistry method was performed to test the Wnt signaling pathway including GSK-3β, β-catenin. Re-sults In the group OGZ and STZ where O-GlcNAc was overexpressed, disappearance of the epithelial layers was de-layed, embryo lips appeared bone defect in bonestaining, compared with the normal group, obvious cell apoptosis has not been found in TUNEL detection. But expression of GSK-3 beta increased in the cytoplasm, expression of beta-catenin nu-cleus reduced, upper lip development of normal side and the low expression of O-GlcNAc in the OGA group were not af-fected. Conclusion Overexpression of O-GlcNAc may lead to a delay of epithelial disappearance which was not caused by the apoptosis but maybe caused by inhibiting Wnt signaling pathway. The process can change related signaling factors including GSK-3 beta, beta-catenin, which may influence the development of upper lip and in the end lead to cleft lip and palate.关键词
Wnt信号通路/氧联乙酰葡萄糖胺/细胞凋亡/鸡胚/上唇Key words
Wnt signaling pathway/O-linked N-acetylglucosamine/Cell apoptosis/Chicken embryo/Upper lip分类
医药卫生引用本文复制引用
朴正国,邹瑞,闫璟,Seok-Kee Baik,Hye-Jin Tak,Sang-Hwy Lee..O-GlcNAc通过抑制Wnt信号通路影响上唇发育的实验研究[J].口腔疾病防治,2016,24(5):273-279,7.基金项目
广东省科技计划项目(2012B032000012) （2012B032000012）
广州市荔湾区科技计划项目(20131215074-2) （20131215074-2）
广州医科大学归国人员基金(2011C02) （2011C02）
