国际口腔医学杂志2016，Vol.43Issue(3)：273-277,5.DOI:10.7518/gjkq.2016.03.005
变异链球菌临床株表面相关蛋白表达差异的初步分析
Differential expression of surface-associated proteins in clinical isolations ofStreptococcus mutans
摘要
Abstract
Objective This study aims to investigate the changes in the expression of surface-associated protein at pH7.0 in Streptococcus mutans isolated from clinical samples. Methods The proteins were extracted from the cells at pH7.0 by using the Homer method. The proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDSPAGE) and 2D gel electrophoresis(2DE) followed by image analysis. The proteins were identified by matrix-assisted laser desorption time of flight(MALDI-TOF) mass spectrometry and computer-assisted protein sequence analysis. Results Image analysis revealed that 14 high expression protein loci and 8 specific protein loci exist in Streptococcus mutans 593. Specifically, pyruvate kinase and adenosine triphosphate-binding cassette transporter were modulated, and glucose transferase was highly modulated in Streptococcus mutans 593. Conclusion The difference in protein expression of the two clinical isolations may indicate their distinct cariogenic characters.关键词
变异链球菌/蛋白质组学/临床分离株/表面相关蛋白Key words
Streptococcus mutans/proteomics/clinical isolation/surface-associated protein分类
生物科学引用本文复制引用
赵兴福,江山,黄晓晶,闫福华..变异链球菌临床株表面相关蛋白表达差异的初步分析[J].国际口腔医学杂志,2016,43(3):273-277,5.基金项目
国家自然科学基金（30500564）This study was supported by the National Natural Science Foundation of China(30500564) （30500564）
