高校化学工程学报2016，Vol.30Issue(2)：410-416,7.DOI:10.3969/j.issn.1003-9015.2016.02.023
一种海洋黑曲霉耐盐内切纤维素酶的分离纯化和酶学性质研究
Purification and Characterization of a Halotolerant Endoglucanase from MarineAspergilla Niger
摘要
Abstract
A marine-derivedAspergillaniger strain was used to produce cellulase by solid state fermentation. The endoglucanase was purified by ammonium sulfate precipitation, ultra-filtration, anion exchange chromatograph and hydrophobic interaction chromatography, and the molecular weight of the endoglucanase was~34 kDa. The specific activity of the purified endoglucanase increased to 557.27 U⋅mg−1 from 37.72 U⋅mg−1 of the crude endoglucanase. The results show that the optimal temperature and pH for better endoglucanase activity are 60℃ and 4.5, respectively. The endoglucanase activity retains up to 87% at 50℃ and 57% at 60℃ after 9 h, and it is stable over a broad pH range. Cu2+ and Fe2+ ions are found as inducers while Mn2+, Ni2+ and Zn2+ significantly inhibit enzyme activity. The endoglucanase activity in 20~60 g⋅L−1NaCl solutions is higher than that in NaCl-free solutions, and the residual activity of about 89% at 20% salt concentration indicates its halotolerance nature. The kinetic constantsKm andVmax determined for endoglucanase with carboxylmethyl cellulose as a substrate are 11.7 mg⋅mL−1 and 943.4 U⋅mg−1, respectively.关键词
内切纤维素酶/分离纯化/耐盐/海洋黑曲霉/纤维素酶Key words
endoglucanase/purification/halotolerant/marineAspergilla niger/cellulase分类
信息技术与安全科学引用本文复制引用
王红兵,王荣柱,陆涛,姚善泾..一种海洋黑曲霉耐盐内切纤维素酶的分离纯化和酶学性质研究[J].高校化学工程学报,2016,30(2):410-416,7.基金项目
国家自然科学基金(21376214)。 （21376214）
