高校化学工程学报2016，Vol.30Issue(2)：417-422,6.DOI:10.3969/j.issn.1003-9015.2016.02.024
重组Pyrococcus furiosus葡聚糖磷酸化酶催化淀粉合成葡萄糖-1-磷酸
Synthesis of Glucose-1-Phosphate from Starch byPyrococcus Furiosus Glucan Phosphorylase
张尧 1吴绵斌 1杨立荣 1林建平1
作者信息
- 1. 浙江大学生物质化工教育部重点实验室，浙江大学化学工程与生物工程学院，浙江杭州 310027
- 折叠
摘要
Abstract
glgP gene encoding glucan phosphorylase (GPase) from thermophilePyrococcus furiosus was codon-optimized and overexpressed inE. coli, and the recombinant GPase was used to synthesize glucose-1-phosphate (G-1-P) from starch. The results show that the GPase exhibits high activity for all kinds of starch studied under the optimal reaction conditions. Corn starch was selected as the raw material for further investigation. When the initial corn starch concentration is 7.5%, and another 2.5% corn starch is added after 16 h, the final production of G-1-P is 250.34 mmol⋅L−1 and the conversion ratio is 65.1% after 48 h reaction, which is similar to that obtained with 10% soluble starch as the substrate (252.9 mmol⋅L−1,65.9%). The results indicate that cheap and widely available corn starch can be used as an alternative of soluble starch for G-1-P production.关键词
葡聚糖磷酸化酶/葡萄糖-1-磷酸/酶催化/淀粉Key words
glucan phosphorylase/glucose-1-phosphate/enzymatic synthesis/starch分类
生物科学引用本文复制引用
张尧,吴绵斌,杨立荣,林建平..重组Pyrococcus furiosus葡聚糖磷酸化酶催化淀粉合成葡萄糖-1-磷酸[J].高校化学工程学报,2016,30(2):417-422,6.
