甘蔗糖业Issue(2)：20-26,7.
重组毕赤酵母发酵生产α-葡聚糖酶的放大研究
Scale-up Fermentation of Recombinant Dextranase Expressed inPichia Pastoris
摘要
Abstract
The high density fermentation of dextranase in the constitutivePichia pastoris expression system scaled up from 6.8 to 5000 L in series was studied. By controlling residual concentration of glycerol and dissolved oxygen combined with pH-stat, multi batches of fermentation were performed. Results showed that the dextranase synthesis under GAP promoter is growth-associated and mainly in exponential phase and stationary phase. Activity of dextranase in 6.8 L, 50 L, 500 L and 5000 L fermenter reached 1253, 1300, 740 and 589 U/mL, respectively. A simple and convenient fermentation method using glycerol as carbon source has been developed, which obviates problems related with methanol safety concerns and is suitable for large scale production.关键词
α-葡聚糖酶/毕赤酵母/组成型表达/发酵放大Key words
Dextranase/Pichia pastoris/Constitutive expression/Fermentation scale-up分类
轻工纺织引用本文复制引用
黄曾慰,吴兆鹏,常国炜,黎志德,张九花,曾练强,梁达奉..重组毕赤酵母发酵生产α-葡聚糖酶的放大研究[J].甘蔗糖业,2016,(2):20-26,7.基金项目
现代农业产业技术体系建设专项资金(CARS-20-4-5)；八桂学者建设工程专项经费 （CARS-20-4-5）
