华北农学报2016，Vol.31Issue(2)：32-37,6.DOI:10.7668/hbnxb.2016.02.007
甘蓝型油菜 TIP4；1基因的克隆与表达分析
Cloning and Expression Analysis of TIP4;1 Gene from Brassica napus
摘要
Abstract
Aquaporins are channel proteins,which located in biological membranes including plasma membrane and tonoplast.It can highly facilitate water transportation across biological membranes.To investigate molecular mechanism of water regulation during seed germination,a tonoplast intrinsic proteins(TIPs)gene fragment TIP4;1 was isolated from Brassica napus seed by RT-PCR.The expression of TIP4;1 gene during seed germination and stresses treatment was analyzed by quantitative Real-time PCR(qRT-PCR).The transcription levels of TIP4;1 was analyzed during germination,and these results showed TIP4;1 expression levels was scarcely detected in dry seed, but was up-regulated during germination as well as early young seedlings.In addition,expression of TIP4;1 in re-sponse to abiotic stress was investigated,and results showed that TIP4;1 gene expression was upregulated under drought,cold and salt stress,suggesting that TIP4;1 may be involved in Brassica napus seed germination and stress response process.Aquaporin regulation mechanism would be further revealed in future,which had realistic signifi-cance for solving drought and salt stress problems.关键词
甘蓝型油菜/水孔蛋白/TIPs/萌发/qRT-PCRKey words
Brassica napus/AQPs/TIPs/Seed germination/qRT-PCR分类
生物科学引用本文复制引用
葛风伟,江一,赵惠新..甘蓝型油菜 TIP4；1基因的克隆与表达分析[J].华北农学报,2016,31(2):32-37,6.基金项目
新疆维吾尔自治区重点实验室“新疆特殊环境物种保护与调控生物学实验室”项目（XJDX1414-2015-07）；新疆师范大学博士科研启动基金项目（XJNUBS1541）；新疆维吾尔自治区自然科学基金项目 （）
