华北农学报2016，Vol.31Issue(2)：52-59,8.DOI:10.7668/hbnxb.2016.02.010
过表达和抑制表达2-Cys Prx 基因烟草的生长、生理特性和叶片光合功能对模拟酸雨的响应
Responses of Growth and Physiological Features,Photosynthetic Function in Overexpression and Inhibition of Expression of Transgenic Tobacco Plants with 2-Cys Prx to the Simulated Acid Rain
摘要
Abstract
The overexpression and inhibition of expression of transgenic tobacco plant with 2-Cys Prx (Longjiang 91 1 ),non transgenic tobacco were chosen to investigate the effects of simulated acid rain on their growth,physiological features and photosynthesis.The results showed that simulated acid rain at pH 4.0 had no sig-nificant effects on the plant height,leaf area and specific leaf weight,but at pH 2.5,the plant height of overexpres-sion and inhibition of expression of transgenic tobacco plant with 2-Cys Prx reduced significantly,With the pH value of the simulated acid rain decreased,chlorophyll content,nitrate reductase activity,soluble protein content,superox-ide dismutase and ascorbate peroxidase activity,net photosynthetic rate(Pn),stomatal conductance(Gs),transpira-tion rate(Tr),the actual photochemical efficiency of PSⅡ(ΦPSⅡ ),photochemical quenching coefficient(qP),po-tential photochemical efficiency of PSⅡ(Fv /Fo)and cell membrane stability index decreased,the non-photochemi-cal quenching(qN)and hydrogen peroxide content increased,but at the same pH value,these above-mentioned in-dexes of overexpression of transgenic tobacco plant with 2-Cys Prx were better than inhibition of expression of trans-genic tobacco plant with 2-Cys Prx and non transgenic tobacco plant.The results suggest that enhanced expression of 2-Cys Prx in tobacco plant can promote the capability of tobacco against acid rain,the capability of utilizing nitrate nitro-gen,and remove of excess H2 O2 while APX activity decreases,and enhance the photosynthetic capability of tobacco.关键词
烟草/2-Cys Prx 基因/模拟酸雨/光合功能Key words
Tobacco/2-Cys Prx gene/Simulated acid rain/Photosynthetic function分类
农业科技引用本文复制引用
代欣,张秀丽,胡举伟,孙广玉..过表达和抑制表达2-Cys Prx 基因烟草的生长、生理特性和叶片光合功能对模拟酸雨的响应[J].华北农学报,2016,31(2):52-59,8.基金项目
哈尔滨市科技创新人才研究专项 （）
