华北农学报2016，Vol.31Issue(2)：60-64,5.DOI:10.7668/hbnxb.2016.02.011
不同浓度铅胁迫对向日葵幼苗蛋白质结构和表达的影响
Effects of Lead Stress on Structure and Expression of Sunflower Seedlings Proteins
摘要
Abstract
In order to explore the molecular mechanism of plumbum tolerance in sunflower,the alterations of sunflower seedlings in protein level under different concentrations of lead stress (0,400,800 mg /L),had been ana-lyzed by FTIR infrared spectrometry and two-dimensional electrophoresis.The results showed that the irregular structurs structure in proteins from the roots,stems,and leaves of sunflower seedlings was significantly sensitive to lead stress compared with other structures.Measurements on the ratio of 1 655 cm -1 /1 637 cm -1 under lead stress indicated that secondary structures of proteins were relatively stable under 400 mg /L of lead solution,while the structures were loosely organized under 800 mg /L.The total proteins of materials mentioned above were extracted by Tris-phenol extraction method.Then 65 same protein spots were found by two-dimensional electrophoresis analysis in comparison with control treatment,more than 9 spots were differences in two times multiples.According to the pro-tein isoelectric and molecular mass to protein databank,speculated that change is more significant with kinase.关键词
向日葵幼苗/铅胁迫/傅立叶红外光谱/SDS-PAGE 电泳/双向电泳Key words
Sunflower seedlings/Lead stress/FTIR/SDS-PAGE electrophoresis/Two-dimensional electrophoresis分类
农业科技引用本文复制引用
吕潇,王聿双,张晓倩,刘海学..不同浓度铅胁迫对向日葵幼苗蛋白质结构和表达的影响[J].华北农学报,2016,31(2):60-64,5.基金项目
天津市大型科学仪器协同开放共享平台建设项目 （）
