华北农学报2016，Vol.31Issue(2)：98-105,8.DOI:10.7668/hbnxb.2016.02.017
T 型细胞质雄性不育小麦 T763A 的败育特点及育性恢复
Abortion Characters and Fertility Restoration of T763A,a Male Sterile Line with T.timopheevi Cytoplasm
摘要
Abstract
T type male sterile wheat line is a valuable material for heterosis study and utilization.In order to make the morphological and cytological characteristics of T763A clear,as well as provide reliable basis for the selec-tion of restorer lines,we used male sterile line T763A,maintainer line 763B and restorer lines(Tm3315B,Tm504B and TP731 B)as materials.The external morphology and pollen grain production(Acetic Acid Magenta,I2 -KI and DAPI)were observed;the karyotype of all the materials was identified by molecular marker technology and A-PAGE,with Chinese spring and Secale cereale L.as control materials.The results showed that the pollen sterility type of male sterile line T763A was stained as spherical abortive;the mature pollen grains were shrunk out of shape and had little inclusion,mean while,the pollen grains were infertile;the sterility of T763A occurred between late u-ninucleate and binucleate stage;all the materials were non 1 B /1 R;the restoring capability of the three restorer lines was high,and among them,Tm504B was the best restorer line of T763A,the reasons for this might in connection with the type of cytoplasm of T763A and the gene counts of the restorer lines.关键词
小麦/细胞质雄性不育/败育特点/核型鉴定/育性恢复Key words
Wheat/Cytoplasmic male sterility/Abortion characters/Karyotyping/Restoration of fertility分类
农业科技引用本文复制引用
段阳,姚盟,蒙立颖,石晓艺,齐智,叶佳丽,闫鹏娇,刘子涵,宋喜悦..T 型细胞质雄性不育小麦 T763A 的败育特点及育性恢复[J].华北农学报,2016,31(2):98-105,8.基金项目
国家自然科学基金项目（31271792）；陕西省农业攻关项目（2014K02-04-01）；西北农林科技大学唐仲英育种基金项目 （）
