华北农学报2016，Vol.31Issue(2)：195-204,10.DOI:10.7668/hbnxb.2016.02.032
GPV 感染扰动雏鹅系统互作网络的研究
Study on Perturbing Systematic Interaction Network of GPV-gosling in an Infectious Context
摘要
Abstract
Perturbing systematic host network for Goose parvovirus (GPV)in natural infection were still un-known.Understanding pathogenic mechanism required that protein,enzymes and isozyme from goslings infected as manifestations of network properties,rather than simply as the result of individual variations.Here blood of experi-mentally GPV infected goslings were examined by biochemical analysis.The results indicated that the activity of pro-tease,Est,POD,SOD,ALP,ADH,Amy,CAT,GPT and LDH from goslings infected GPV were higher 41% /63%, 30% /53%,50% /14%,36% /73%,156% /142%,1005% /22%,36% /26%,13% /51%,60% /244%,289% /139%than that of control group respectively.The content of IgG and IgM from goslings infected GPV were lower 50%, 40% than that of control group respectively,but protein was higher 50%.The isozyme of Est,SOD,ADH and Amy appeared 2,2,1 ,3 new enzyme band respectively,but deleted 2 slow-zone Est isozyme band in the serum of goslings infected Goose parvovirus.The isozyme of Est,POD,SOD and Amy appeared 1 ,2,1 ,2 new enzyme band in the blood corpuscle of goslings infected Goose parvovirus respectively.The isozyme of CAT,LDH,GPT and ALP ap-peared 7,1 ,3,3 variation band in the blood of goslings infected GPV respectively.The isozyme of CAT and ADH defected fast-zone and slow-zone,fast-zone band in the blood corpuscle of goslings infected Goose parvovirus respec-tively.The isozyme of ALP defected slow-zone and fast-zone band in the serum and blood corpuscle of goslings in-fected GPV respectively.At the same time,it also showed that the 59 kDa protein band was a common feature of IgM/G in gosling,and IgG deleted still 258,36 kDa protein in the blood of goslings infected Goose parvovirus.The protein were increased by 131 ,86,43,33 kDa and 144,104,58,53 kDa band in the serum and blood corpuscle of goslings infected Goose parvovirus respectively.There were deleted 1 slow-zone zymoprotein band in the serum of goslings infected GPV,and increased 2 slow-zone zymoprotein band in the blood corpuscle of goslings infected GPV.Those suggested that the interaction of GPV stress and host protein /enzymes and isozyme gene expression, through unique host dynamic balance of metabolic network directly or indirectly regulated cell disturbance suscepti-ble performance.关键词
雏鹅/鹅细小病毒/蛋白 /酶/同工酶/代谢网络Key words
Gosling/Goose parvovirus/Protein /Enzymes/Isozyme/Metabolic network分类
农业科技引用本文复制引用
朱新产,王倩文,朱峰伟,杨丽金..GPV 感染扰动雏鹅系统互作网络的研究[J].华北农学报,2016,31(2):195-204,10.基金项目
山东省自然科学基金项目（ZR2011 CM008）；青岛市科技发展计划项目（12-1-4-5-（2）-jch） （）
