华北农学报2016，Vol.31Issue(2)：211-217,7.DOI:10.7668/hbnxb.2016.02.034
山东葫芦科蔬菜上病毒病种类检测及黄瓜花叶病毒分离物的亚组鉴定
Detection of Viruses Infecting Cucurbit Vegetables in Shandong and Identification of Subgroup of the Cucumber mosaic virus Isolates
摘要
Abstract
In order to detect and identify the viral causing agent that infecting cucurbit vegetable plants in Shandong,867 cucurbit plant samples of suspected viral diseases from 1 1 vegetable planting areas in Shandong were collected during July to August,2014.9 primer pairs specific to Cucumber mosaic virus(CMV),Cucumber green mottle mosaic virus(CGMMV),Watermelon mosaic virus(WMV),Tobacco mosaic virus(TMV),Zucchini yellow mo-saic virus(ZYMV),Squash mosaic virus(SqMV),Melon yellow spot virus(MYSV),Cucurbit chlorotic yellows virus (CCYV),Prunus necrotic ringspot ilarvirus(PNRSV)and Melon necrotic spot virus(MNSV)were used respectively to perform PCR detection.The results showed that viruses carried rate was 34.8%,10.4%,20.0%,41 .7%,27.0%, 7.8%,2.6%,1 .7%,0 and 0.9% respectively.The natural infection of these viral diseases except PNRSV in Shandong was confirmed,and the incidence of CMV and TMV were higher than others.Two or more than two viruses mixed infections were also very common in field.At the same time,those CMV positive samples from 1 1 regions were selected for subgroup classification.Coat protein (CP)gene and the whole RNA3 nucleotide sequences were determined.Sequence alignment and phylogenetic analyses were performed.The results showed that the CMV iso-lates of cucurbit vegetables in Shandong area were all members of CMV subgroup IB with the highest similarity to a South Korea isolate As(AF013291 ),no other strains were found.关键词
瓜类病毒病/黄瓜花叶病毒/RT-PCR/亚组Key words
Cucurbit viral disease/Cucumber mosaic virus/RT-PCR/Subgroup分类
农业科技引用本文复制引用
孙晓辉,王树森,高利利,乔宁,刘永光,赵静,竺晓平..山东葫芦科蔬菜上病毒病种类检测及黄瓜花叶病毒分离物的亚组鉴定[J].华北农学报,2016,31(2):211-217,7.基金项目
国家公益性（农业）行业科研专项资助项目（201303028）；山东省科技发展计划资助项目（2014GNC111008）；山东省自然科学基金项目 （）
