华北农学报2016，Vol.31Issue(2)：231-238,8.DOI:10.7668/hbnxb.2016.02.037
犬瘟热病毒疫苗免疫逃逸株的血凝素基因变异研究
Sequence Analysis of the Hemagglutinin Gene of Canine distemper virus from Vaccinated Dogs,Minks and Foxes
摘要
Abstract
In order to understand whether new genotype emerged,we detected Canine distemper virus(CDV)in immunized dogs,breeding foxes and minks in different regions by amplification of nucleocapsid(N)and hemaggluti-nin(H)gene.13 strains from CDV-positive cases(8 minks,2 foxes and 3 dogs)were sequenced.The results showed that the 13 Chinese strains shared 99.1 %-100.0% nucleotide identity.Compared with those of other Chinese CDV strains isolated before 2012,four consistent amino acid changes,P200S,M263I,I542N and Y549H,were observed in the H coding sequence of CDV isolated from vaccinated animals,which contribute to an additional N-glycosylation site and antigenic epitope.All of the strains obtained from 13 samples were characterized as genotype Asia-1 accord-ing to the sequence analysis of the complete H gene,which was the sole genotype in minks in China.These findings provide a comprehensive understanding of current immune failure and the molecular epidemiology of CDV in China.关键词
犬瘟热病毒/血凝素基因/免疫失败Key words
Canine distemper virus/Hemagglutinin gene(H)/Vaccination failure分类
农业科技引用本文复制引用
黄娟,隋晓梅,王怡男,单虎..犬瘟热病毒疫苗免疫逃逸株的血凝素基因变异研究[J].华北农学报,2016,31(2):231-238,8.基金项目
国家自然科学基金资助项目 （）
