华南农业大学学报2016，Vol.37Issue(3)：73-81,9.DOI:10.7671/j.issn.1001-411X.2016.03.011
青枯菌侵染不同抗病烟草品种的防御性酶活性及代谢组分差异分析
Differences of defensive enzyme activities and metabolites between resistant and susceptible tobacco cultivars infected by Ralstonia solanacearum
摘要
Abstract
Objective] In order to provide the biochemical and metabolic basis of tobacco breeding for disease resistance and resistance identification .[Method]Resistant ( Yueyan 97 ) and susceptible ( Changbohuang ) tobacco cultivars were inoculated with Ralstonia solanacearum by trunk injection . Defensive enzyme activities and metabolites were measured by using colorimetric method and GC-MS.[Result]The activities of phenylanine ammonia lyase ( PAL) , superoxide dismutase ( SOD) , peroxidase (POD) and polyphenol oxidase (PPO) in the resistant cultivar ‘Yueyan 97’ were higher than those in the susceptible cultivar ‘Changbohuang ’ with the same inoculation treatment .The PAL activities of Yueyan 97 and Changbohuang were higher than that in control on the seventh and fifth days after inocula -tion, respectively .The PAL activities of both cultivars were lower compared to control at any other time . For both cultivars, SOD, POD and PPO activities increased compared to control in the earlier days , but were lower compared to control during the later time period .The types of POD and PPO isozymes in the resistant cultivar ‘Yueyan 97 ’ were higher than those in the susceptible cultivar ‘Changbohuang ’ .The width and color of isozyme bands of Yueyan 97 were enhanced at 3 d after inoculation , while those of Changbohuang were not enhanced .It suggested that the resistant cultivar could quickly respond to exter-nal stimuli and accelerate the formation of defense-related substances .Detection of metabolites showed that the relative contents of some substances in Yueyan 97 such as myo-inositol , nicotine , malic acid , and L-threonine were higher compared to control , but in Changbohuang were lower compared to control , suggesting that cultivar differences in resistance against R.solanacearum might be related to these sub-stances .[Conclusion] These four defensive enzyme activities and the contents of the above metabolites could be used as biochemical indexes for evaluating tobacco resistance against R.solanacearum.关键词
烟草/青枯雷尔菌/防御性酶/酶活性/同工酶/代谢组分Key words
tobacco/Ralstonia solanacearum/defensive enzyme/enzyme activity/isozyme/metabolite分类
农业科技引用本文复制引用
周星洋,张功营,董丽红,杨恩兰,万树青..青枯菌侵染不同抗病烟草品种的防御性酶活性及代谢组分差异分析[J].华南农业大学学报,2016,37(3):73-81,9.基金项目
广东省烟草专卖局（公司）科技项目 （）
