海洋科学2016，Vol.40Issue(1)：1-9,9.DOI:10.11759/hykx20140429002
基于线粒体COI基因的毛蚶群体遗传多样性
Genetic diversity of different populations ofScapharca sub-crenata based on mitochondrial COI gene
摘要
Abstract
General PCR technology was used for the amplification of mitochondrial COI gene partial sequences of four groups ofScapharca subcrenata (Dalian, Rushan, Yantai, and Zhoushan). A total of 38 sequences from different populations were determined and analyzed. The results are as follows: (a) After excluding both sides of the inaccurate sequences, 38 COI gene partial sequences of S. subcrenata, 625 bp each, were eventually identified, among which 30 haploid types were de-tected. (b) Based on partial COI gene sequence analysis of the four groups of S. subcrenata, the total variation loci, haploid type diversity index S, nucleotide diversity index Pi, and average nucleotide difference index were 301, 241, 0.15048, and 92.242, respectively. (c) Cluster analysis showed that ark shells from three groups, Rushan, Dalian, and Yantai, had a high degree of genetic diversity, and they clustered across, without the obvious characteristics of the groups. However, ark shells from the Zhoushan group gathered themselves together, apart from the other three groups obviously. The genetic diversity index of Zhoushan group was much lower than that of other groups. (d) Mitochondrial COI gene could not separate the ark shells from Rushan, Dalian, and Yantai but can be a marker for Zhoushan populations ofS. subcrenata, which provides more basic data for the application of mitochondrial COI gene in population genetics.关键词
毛蚶(Scapharca subcrenata)/COI基因/群体/遗传多样性Key words
Scapharca subcrenata/COI gene/population/diversity分类
农业科技引用本文复制引用
田吉腾,侯丫,刘志鸿,杨爱国,吴彪,周丽青,董春光..基于线粒体COI基因的毛蚶群体遗传多样性[J].海洋科学,2016,40(1):1-9,9.基金项目
国家科技部基础工作专项(2013FY110700) （2013FY110700）
黄海水产研究所级基本科研业务费资助(20603022013032)@@@@ National Basic Research Special Foundation of China (2013FY110700) （20603022013032）
Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institutes (20603022013032) （20603022013032）
