海洋科学2016，Vol.40Issue(1)：10-17,8.DOI:10.11759//hykx20140803003
单体牡蛎诱导变态的研究
The induction of metamorphosis in cultchless oysters(Crass-ostrea angulata and Crassostrea gigas)
摘要
Abstract
To obtain cultchless oysters with a regular shell shape and uniform size, epinephrine (EPI) was applied to Crassostrea angulata andCrassostrea gigas eye-larvae to induce cultchless metamorphosis. We examined the effect of larval density, concentration of EPI, and length of exposure to EPI to determine optimum levels for each species. ForC. angulata, we found that the optimum concentration of EPI was 5×10−4mol/L for a 24 h treatment (metamorphic rate = 72.8%) and the optimum treatment time at this concentration was 12 h (metamorphic rate = 82.7%). In contrast, inC. gigas, the optimum concentration of EPI was 5×10−5 mol/L over 6 h (metamorphic rate = 53.2%), and the optimum treatment time at this concentration was 8 h (maximum metamorphic rate = 56.8%). However, there was no significant dif-ference between EPI treatments at larval densities < 80 individuals/mL. The growth ofC. gigas was also determined over the following days, which showed that shell length, shell height, and larval survival rate was higher in the treated groups than those in the control groups. Therefore, EPI can facilitate larval metamorphosis and improve their survival ability.关键词
葡萄牙牡蛎(Crassostrea angulata)/长牡蛎(Crassostrea gigas)/单体牡蛎/不固着变态Key words
Crassostrea angulata/Crassostrea gigas/cultchless oyster/metamorphosis without settlement分类
农业科技引用本文复制引用
陈亨,方军,滕爽爽,肖国强,张炯明,柴雪良..单体牡蛎诱导变态的研究[J].海洋科学,2016,40(1):10-17,8.基金项目
温州市水产增养殖技术创新团队项目(C20120004-3) （C20120004-3）
国家贝类产业技术体系试验站项目(CARS-48) （CARS-48）
水产种质资源平台运行服务项目(2014DKA30470) （2014DKA30470）
浙江省科技计划项目(2013F50016)@@@@Mariculture Technology Innovation Team Project III of Wen-Zhou City(C20120004-3) （2013F50016）
The test station of national shelfish-industry tech-nology system (CARS-48) （CARS-48）
The operation service platform of aquatic germ-plasm resources(2014DKA30470) （2014DKA30470）
Scientific technology project of Zhejiang Province(2013F50016) （2013F50016）
