海洋科学2016，Vol.40Issue(1)：18-24,7.DOI:10.11759/hykx20150319001
运用分子标记辅助构建海带核心种质资源
Establishment of core collection forSaccharina japonicausing molecular markers
摘要
Abstract
Kelp is a large alga with an important economic and ecological value. The gametophytes are the preserved materials for kelp germplasm resources, which are also used for kelp crossbreeding and seedling. For an effective management and evaluation of kelp germplasm resources, a core collection of germplasm was screened. Genetic evaluation of 28 gametophytes of “Zaohoucheng” variety was conducted by RAPD molecular marker technology, using the UPGMA clustering method, based on the genetic similarity coefficient of grouping, simple percentage sampling (25%, 35%, 50%, and 35%), and analysis and calculation of genetic diversity parameters of different sampling rates, combined with the growth status and proportion of males and females. The core collection at the sampling rate of 50% was relatively reasonable. These results laid the foundation for screening and genetic evaluation of core germplasm of kelp varieties (or lines).关键词
海带(Saccharina japonica)/RAPD分子标记/核心种质/遗传多样性Key words
Saccharina japonica/RAPD molecular markers/core germplasm/genetic diversity分类
农业科技引用本文复制引用
崔翠菊,田鑫,李言,刘延岭,李晓捷,罗世菊,武瑞娜,王利芹,张壮志,孙娟..运用分子标记辅助构建海带核心种质资源[J].海洋科学,2016,40(1):18-24,7.基金项目
国家科技支撑计划项目(2012BAD55G01) （2012BAD55G01）
国家高技术研究发展计划(863计划)项目(2012AA10A406) （863计划）
烟台市科技发展计划项目(2013LGS002)[Fund programs：National Supporting Program of Science and Technology of China (2012BAD55G01) （2013LGS002）
National High Technology Research and Development Program (863 Program) of China (2012AA10A406), and Municipal Science and Technology Research and Development Project of Yantai (2013LGS002)] （863 Program）
