海洋科学2016，Vol.40Issue(2)：20-25,6.DOI:10.11759/hykx20141018001
长江口泥螺群体遗传多样性的AFLP分析
Amplified fragment length polymorphism analysis of the ge-netic diversity ofBullacta exaratain the Yangtze estuary
摘要
Abstract
In this study, the genetic diversity ofBullacta exarata from four populations (Qidong, Chongming Dongtan, Jiuduansha, and Nanhui) in the Yangtze estuary were investigated based on amplified fragment length polymorphism (AFLP) profiles. The four AFLP primer combinations produced 577 AFLP loci and the percent loci polymorphic values for QD, CM, JD, and NH were 78.2%, 56.0%, 58.4%, and 57.9%, respectively. The total gene diversity index was 0.2218, indicating a high level of genetic diversity and over 96% of the genetic variation was because of individuals within populations. Genetic differentiations among populations were very low (0.0000–0.0404) and the Nei’s genetic distance among four populations varied from 0.0000 to 0.0124. In addition, structure analysis indicated thatB. exarata popula-tions originated from three genetic groups and strong gene flow existed among populations. The ability ofB. exarata to disperse in the open sea could be the cause of the slight genetic variation in the studied areas.关键词
泥螺/AFLP/遗传多样性Key words
Bullacta exarata/amplified fragment length polymorphism/genetic diversity分类
农业科技引用本文复制引用
杨梅,徐嘉伟,王成辉,张文驹..长江口泥螺群体遗传多样性的AFLP分析[J].海洋科学,2016,40(2):20-25,6.基金项目
长江大学湿地生态与农业利用教育部工程研究中心开放基金(KF201411)@@@@ The project was financially supported by open fund of Engi-neering research of ecology and agricultural use of wetland, ministry of education (KF201411)。 （KF201411）
