江西农业大学学报2016，Vol.38Issue(2)：248-254,7.DOI:10.13836/j.jjau.2016035
油菜蕾薹期和花期菌核病接种方法比较
Comparison of Methods for Resistance Screening aganist Sclerotinia sclerotiorum in Brassica napus
摘要
Abstract
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is a major disease in oilseed rape.To es⁃tablish a rapid and effective method for evaluation of resistance in Brassica napus to S. sclerotiorum four artifi⁃cial inoculation methods and five genotypes of Brassica napus were used in this study. The four methods were detached leaf inoculation ( DLI) ,detached stem inoculation with toothpick ( DSI) ,stem inoculation with tooth⁃pick in vivo (SIT),and petiole inoculation with pipette tip in vivo (PIT).No significant correlations were de⁃tected between detached leaf inoculation and the other three methods.The lesion length of PIT at 11 dpi (days post⁃inoculation) and 19 dpi showed significant ( P<0.05) correlations with lesions of DSI at 3dpi and 7 dpi (r=0.866 and 0.821),and highly significant (P<0.01) correlations with lesions of SIT at 7 dpi and 15 dpi (r=0.952 and 0.987).These results suggest that the petiole inoculation with pipette tip is a convenient and re⁃liable method for screening the resistance against S.sclerotiorum in B.napus.关键词
油菜/核盘菌/人工接种/叶柄接种Key words
oilseed rape/Sclerotinia sclerotiorum/artificial inoculation/petiole inoculation分类
农业科技引用本文复制引用
任莉,陈坤荣,曾令益,刘凡,徐理,方小平..油菜蕾薹期和花期菌核病接种方法比较[J].江西农业大学学报,2016,38(2):248-254,7.基金项目
湖北省自然科学基金项目（2013CFB426）、国家公益性行业（农业）科研专项（201103016）和国家油菜产业技术体系资助项目（ CARS-13） Project supported by the Natural Science Foundation of Hubei Province，China （2013CFB426），the Special Fund for Agro-scientific Research in the Public Interest （201103016） and the Industry Technology System of Rape-seed in China （）
