江西农业大学学报2016，Vol.38Issue(2)：381-385,392,6.DOI:10.13836/j.jjau.2016054
基于DNA条形码的我国吴茱萸主要栽培种遗传分析和分子鉴别研究
A Study on Genetic Relationship Analysis and Molecular Indenfication Based on DNA Barcoding of the Main Cultivated Species of Evodia rutaecarpa in China
摘要
Abstract
ITS2 and psbA⁃trnH were used to analyze the genetic background of Evodia rutaecarpa ’ s main cultivated species in China and identify them. ITS2 sequences were 223 bp and 7 differential sites were ob⁃tained inculding two specificity sites.The total length of psbA⁃trnH was 419 bp including three specificity sites. The system clustering tree was constructed on the basis of ITS2 sequences. The result showed that the genetic background distinction between Evodia rutaecarpa and Evodia ·rutaecarpa ( Juss.·) Benth ·var ·officinalis (Dode ) Huang,E. rutaecarpa (Juss.) Benth.var. bodinieri (Dode) Huang was obvious and the relationship between Evodia rutaecarpa (Juss) Benth and was closer.And grafting made the big variation in genetic back⁃ground.The sequence specificity sites of this test could be used in accurate and effective identification of genu⁃ine and fake Evodia rutaecarpa.关键词
吴茱萸/DNA条形码/遗传分析/分子鉴别Key words
Evodia rutaecarpa/DNA barcoding/genetic analysis/molecular identification分类
农业科技引用本文复制引用
吴波,罗光明,李永波,曾金祥..基于DNA条形码的我国吴茱萸主要栽培种遗传分析和分子鉴别研究[J].江西农业大学学报,2016,38(2):381-385,392,6.基金项目
江西省卫生和计划生育委员会科技项目（2013A007） Project supported by Health and Family Planning Commission of Jiangxi Province （）
