检验医学与临床2016，Vol.13Issue(9)：1164-1166,3.DOI:10.3969/j.issn.1672-9455.2016.09.005
标本保存时间及温度对流式细胞仪检测非健康人外周血淋巴细胞亚群影响研究
The influence of storage time and temperature on flow cytometry analysis of patients′peripheral blood lymphocyte sub-sets
摘要
Abstract
Objective To evaluate the effect of sample storage time and temperature on the analysis of periph‐eral blood lymphocyte subsets in non‐healthy human by flow cytometry .Methods Group one involved 27 patients with multiple diseases .Whole blood was collected for flow cytometry analysis within 6 h(group A) ,at 72 h(group B) and 120 h(group C) .Group two involved 18 patients with multiple diseases .Samples were tested in 3 groups:group D ,the samples were processed and tested within 6 hours since it collected;group E ,samples were processed within 6 hours ,and were stored at 4 ℃ for 24 hours before test ;group F ,samples were stored at 25 ℃ for 36 hours ,and then were processed and tested .Results Compared with group A in group one ,the data showed lower CD3+ cells ,CD3+CD4+ cells(P<0 .05)and higher CD19+ cells(P<0 .05) ,CD16+ CD56+ cells(P< 0 .05)of group B and group C . CD3+CD8+ cells were higher in group B than group A(P<0 .05) .In group two ,there was no significant difference between group D ,group E and group F(P>0 .05) .Conclusion The results of peripheral blood lymphocyte subsets of patients analyzed by flow cytometry are stable within 36 hours after the sample collection .关键词
流式细胞术/淋巴细胞亚群/储存温度/储存时间Key words
flow cytometry/lymphocyte subsets/storage temperature/storage time引用本文复制引用
方芳,陈柯霖,刘淑静,周金,张国军,康熙雄..标本保存时间及温度对流式细胞仪检测非健康人外周血淋巴细胞亚群影响研究[J].检验医学与临床,2016,13(9):1164-1166,3.基金项目
北京市卫生系统高层次卫生技术人才培养计划项目（2013-3-052）。 （）
