林产化学与工业2016，Vol.36Issue(2)：51-56,6.DOI:10.3969/j.issn.0253-2417.2016.02.008
碱预处理玉米芯生物转化L-乳酸
Production of L-Lactic Acid by Bioconversion of Alkali Pretreated Corncob
摘要
Abstract
Corncob pretreated by alkali was used as renewable raw material for L-lactic acid production by Rhizopus oryzae. The processes of separate saccharification and fermentation ( SHF) and simultaneous saccharification and fermentation ( SSF) were studied. The results showed that the enzymatic hydrolysis performance of washed alkali pretreated corncob was better than that of unwashed alkali pretreated corncob. Water-washing process could enhance the fermentability of alkali pretreated corncob. The washed alkali pretreated corncob hydrolysate with 31. 84 g/L glucose and 6. 38 g/L xylose was converted to 14. 65 g/L of L-lactic acid which was equivalent to a yield of 0. 29 g/g dry biomass with SHF process at 40 ℃ for 48 h. In contrast, a higher L-lactic acid yield (0. 44 g/g dry biomass) was achieved at 40 ℃ for 36 h, when the substrate concentration of 50 g/L washed alkali pretreated corncob was used in SFF process.关键词
米根霉/碱预处理玉米芯/L-乳酸/分步糖化发酵/同步糖化发酵Key words
Rhizopus oryzae/alkali pretreated corncob/L-lactic acid/separate saccharification and fermentation/simultaneous saccharification and fermentation分类
化学化工引用本文复制引用
张丽,陈晓佩,李鑫,余世袁,勇强..碱预处理玉米芯生物转化L-乳酸[J].林产化学与工业,2016,36(2):51-56,6.基金项目
“十二五”国家科技支撑计划资助(2015BAD15B09) （2015BAD15B09）
江苏高校自然科学研究重大项目(14KJA220003) （14KJA220003）
江苏省普通高校研究生科研创新计划项目(CXZZ13_0544) （CXZZ13_0544）
