临床口腔医学杂志2016，Vol.32Issue(5)：259-261,3.DOI:10.3969/j.issn.1003-1634.2016.05.002
干扰细胞信号转导抑制因子1表达对人口腔癌细胞生物学功能的影响
Gene interference of suppressor of cytokine signaling-1 on the biological functions of human oral cancer cell
朱郁文 1马壮 1张胜 1姚建1
作者信息
- 1. 株洲市中心医院口腔科 湖南 株洲 412007
- 折叠
摘要
Abstract
Objective:To investigate the effects of cell proliferation, cell cycle and invasion ability of human oral cancer cell after suppressor of cytokine signaling-1 (SOCS1) gene interference. Method:Western blotting, real-time PCR were used to verify the down regulation of SOCS1 human oral cancer cell line (KB) after transfection; and the cell proliferation rate was detected by MTT assay; the invasive ability of KB cells in vitro was evaluated by Transwell migration assay; flow cytometry was used to detect the cell cycle. Result:Results of RT-PCR and western blotting showed that the expression levels of SOCS1 mRNA and protein were significantly decreased in small interfering SOCS1 transfected KB cells. 72 hours after the SOCS1 silencing, the cell proliferation rate dropped significantly compared with that of control group (P<0.05). After the silence of SOCS1, the invasive ability of KB cells in vitro was decreased (P<0.05). The cell cycle arrest was promoted at the G0/G1 phase, but the percentage of cells in S phase and G2/M decreased compared to that of control groups (P<0.05). Conclusion:Inhibition of SOCS1 gene expression decreases the proliferation ability, and invasion ability in vitro of human oral cancer cell line KB, and inhibits cell division.关键词
人口腔癌/细胞因子信号转导蛋白抑制因子1/基因干扰/细胞增殖/侵袭Key words
Human oral cancer/Suppressor of cytokine signaling protein 1/Gene interference/Cell proliferation/Invasion分类
生物科学引用本文复制引用
朱郁文,马壮,张胜,姚建..干扰细胞信号转导抑制因子1表达对人口腔癌细胞生物学功能的影响[J].临床口腔医学杂志,2016,32(5):259-261,3.
