临床口腔医学杂志2016，Vol.32Issue(5)：269-271,272,4.DOI:10.3969/j.issn.1003-1634.2016.05.006
Transwell小室环境下小鼠不同器官微环境对人舌癌细胞生长的影响
The effect of different organs microenvironments of mice on the growth of human tongue cancer cells in a Transwell cell insert
摘要
Abstract
Objective:To investigate the effect of bone marrow and lymph node microenvironment on the growth of hu-man tongue squamous carcinoma cell line SCC-9. Method:Transwell cell insert was uesd to establish the microenvironment and SCC-9 co-cultured model in vitro. The experiment was divided into 5 groups: SCC-9 cultured alone group (control group), SCC-9 and Balb/c mice bone marrow co-cultured group, SCC-9 and Balb/c mice lymph nodes co-cultured group, SCC-9 and Balb/c nude mice bone marrow co-cultured group, SCC-9 and Balb/c nude mice lymph nodes co-cultured group. The number of SCC-9 was counted by direct counting method at 24 h, 48 h, 72 h, 96 h, 120 h, 144 h. Result:The different microenvironments and SCC-9 co-cultured model in vitro was established by Transwell cell insert successfully. The proliferation of SCC-9 in Balb/c nude mice lymph node microenvironment was higher than those of the other four groups(P<0.05). There is no statistic difference among Balb/c mice bone marrow, nude mice bone marrow and nude mice lymph nodes microenvironment(P>0.05),in which the SCC-9 proliferations were lower than the control group(P<0.05). Conclusion:Dif-ferent microenvironments may control the proliferation and dormancy of tumor cell by different tumor dormancy mechanisms, and cause the passive targeted lymphatic metastasis.关键词
Transwell小室/共培养/舌癌细胞/微环境/肿瘤休眠Key words
Transwell cell insert/co-cultured/Tongue cancer cells/Microenvironment/Tumour dormancy分类
生物科学引用本文复制引用
郭梦竹,牟云,于大海,梁飞新,温琦涛,王振瑞..Transwell小室环境下小鼠不同器官微环境对人舌癌细胞生长的影响[J].临床口腔医学杂志,2016,32(5):269-271,272,4.基金项目
国家自然基金（81360407）广西自然科学基金 （）
