临床口腔医学杂志2016，Vol.32Issue(5)：281-283,284,4.DOI:10.3969/j.issn.1003-1634.2016.05.010
复合聚己内酯静电纺丝膜的制备与细胞相容性的研究
Fabrication and cytocompatibility of electrospinning polycaprolactone scaffolds with different components
陈静 1高凌云 1王愉惠 1刘月华1
作者信息
- 1. 同济大学口腔医学院和附属口腔医院正畸科·上海牙组织修复与再生工程技术研究中心 上海 200072
- 折叠
摘要
Abstract
Objective:The study aimed to fabricate electrospinning membranes with PCL,COL and HA,and test their biocompatibility with periodontal ligament cells (PDLCs). Method:Three electrospinning membranes were fabricated with different spinning solution composition of PCL, PCL+5 %COL and PCL+5 %COL+1 %HA, respectively. The membrane morphology was observed through scan electron microscope and the hydrophilic performance was tested by contact angles. MTT was applied to investigate the proliferation of PDLCs seeded on the membranes and the viability was measured by live and dead staining method. DAPI staining was used to observe the distribution of PDLCs co-cultured with membrane. Re-sult:All the three types of membranes were fabricated with uniform nano-fibers. The fibers of PCL+5 %COL membrane ex-hibited concave surface and that of PCL+5 %COL+ 1 %HA membrane raised nodular surface with smooth and continuous surface. PCL+5%COL+1%HA membrane had the smallest contact Angles rank among the three membranes. The prolifera-tion and viability of PDLCs on the PCL+5 %COL+ 1 %HA membrane were significantly higher than those on other two membranes(P<0.01). Additionally,cells on the PCL+5%COL+1%HA membrane was more intensive than other two groups (P<0.01). Conclusion:The composition solution of COL,HA and PCL can change the morphology of electrospinning mem-branes. Among them,the PCL+5%COL+1%HA electrospinning membrane suggested good biocompatibility.关键词
静电纺丝/牙周膜细胞/微观形貌/细胞相容性Key words
Electrospinning/Periodontal ligament cells/Morphology/Cell compatibility分类
医药卫生引用本文复制引用
陈静,高凌云,王愉惠,刘月华..复合聚己内酯静电纺丝膜的制备与细胞相容性的研究[J].临床口腔医学杂志,2016,32(5):281-283,284,4.
