临床小儿外科杂志2016，Vol.15Issue(2)：150-155,6.DOI:10.3969/j.issn.1671-6353.2016.02.014
MiR-483-3P下调RIOK3蛋白表达促进神经母细胞瘤的增殖和迁移
MiR-483 -3P down regulate RIOK3 expression and promote the proliferation and migration ability of human neuroblastoma cells
摘要
Abstract
Objetive To explore the influence of microRNA—483 —3P (miR—483 —3P)on neuroblastoma cells'proliferation,invasion and migration abilities,and predict target genes for miR —483 —3P,and also to dis-cuss its impact on the target gene. Methods miRNA array results showed that miR—483 —3P ,which has the most significant difference in the changed miRNAS,was upregulated in neuroblastoma.Under the use of cationic liposomes LipofectamineTM2000 ,chemically synthesized miR—483 —3P inhibitor or a negative control sequence of miR—483 —3P inhibitor were transiently transfected into human neuroblastoma SH—SY—5Y cell lines.The ex-pression of miR—483 —3P was detected by using quantitative Real-time PCR technique.The proliferation and mi-gration of neuroblastoma cells were examined in vitro by CCK—8 and transwell experiment assay.The bioinfor-matics software was used to predict target genes of miR—483 —3P and luciferase reporter gene detection experi-ments,Western blot experiments were proformed to validate the target gene. Results Compared with adjacent none-tumor tissues,miR—483 —3P was upregulated in neuroblastoma tissues(P <0.01).Compared with nega-tive control,the miR—483 —3P expression was decreased after miRNA inhibitor transfection(P <0.01),the pro-liferation and migration of cells in vitro are all decreased(P <0.05).Bioinformatics software predicted RIOK3 is one of the target genes of miR—483 —3P,when cells transfected with miR—483 —3P inhibitor the luciferase ac-tivity would increased(P <0.01).Compared with the negative control group,when cells transfected with miR—483 —3P inhibitor at the protein level RIOK3 would increased(P <0.01). Conclusion RIOK3 is one of the target genes of miR—483 —3P,also miR—483 —3P can down regulate the expression of RIOK3 and promote the proliferation and migration abilities of neuroblastoma cell.关键词
神经母细胞瘤/miR-483 -3P/细胞增殖/侵袭/靶基因Key words
Neuroblastoma/miR-483 -3P/proliferation/invasion/target gene引用本文复制引用
李晓,鹿洪亭,董蒨,郝希伟,赵静,刘铭..MiR-483-3P下调RIOK3蛋白表达促进神经母细胞瘤的增殖和迁移[J].临床小儿外科杂志,2016,15(2):150-155,6.基金项目
1，国家自然科学基金资助项目（项目号81272986）；2，山东省高等学校科技计划资助项目（项目号JIILF58）；3，青岛市科技计划基础研究资助项目（项目号10-3-4-3-R-jch）。 （）
