国际眼科杂志2016，Vol.16Issue(5)：822-825,4.DOI:10.3980/j.issn.1672-5123.2016.5.07
氧化损伤诱导的人晶状体上皮细胞系基因表达谱的差异和表型改变
Discrepant gene expression profile and phenotype changes in human lens epithelial cells after oxidative damage
摘要
Abstract
AlM:To explore the discrepant gene expression profiles and the related phenotype changes in human lens epithelial cells( HLEC) after oxidative stimulation. <br> METHODS:Human lens epithelial cell line ( HLE-B3 ) were cultured in normal condition or with H2 O2 for 24h. Total RNA were extracted for gene expression profiling assay and gene ontology analysis was performed for the significantly up-regulated genes using bioinformational database DAVlD. The elevated expressions of up -regulated genes in HLEC after oxidative stimulation were confirmed by RT-qPCR. The apoptosis of HLEC induced by oxidative damage was detected using 3 - ( 4, 5 -dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide( MTT) assay and flow cytometry. <br> RESULTS: Gene expression profiling assay demonstrated that 367 genes were up-regulated in HLEC after oxidative stimulation. These genes were enriched in 310 biological processes mainly associated with p53 -related signaling pathways, apoptosis, programed cell death and etc. Six genes mainly pro- or anti-apoptotic, including BCL2A1, TP53l3, FAS, ZMAT3, DDB2 and BCL2L1, were confirmed to be up-regulated by oxidative stimulation using RT-qPCR(P<0. 05). Results of MTT assay and flow cytometry showed that apoptosis of HLEC gradually appeared after cells were treated with H2 O2 and became the main consequence of oxidative stimulation. <br> CONCLUSlON:Oxidative stimulation can induce up-regulation of proapoptotic genes and lead to apoptosis of HLEC, even though antiapoptotic genes can also be promoted.关键词
人晶状体上皮细胞/氧化损伤/表达谱芯片/细胞凋亡Key words
human lens epithelial cells/oxidative damage/gene expression profile/apoptosis引用本文复制引用
梅林,王嵩,闫博,杨安钢,赵晶,严宏..氧化损伤诱导的人晶状体上皮细胞系基因表达谱的差异和表型改变[J].国际眼科杂志,2016,16(5):822-825,4.基金项目
国家自然科学基金(No.81370997)@@@@National Natural Science Foundation of China (No.81370997) （No.81370997）
