国际眼科杂志2016，Vol.16Issue(5)：826-828,3.DOI:10.3980/j.issn.1672-5123.2016.5.08
番茄红素对紫外线诱导人晶状体上皮细胞氧化损伤的保护作用
Protective effect of lycopene for oxidative damage in human lens epithelial cells induced by UV
孙敬文 1郝静1
作者信息
- 1. 150060 中国黑龙江省哈尔滨市，中航工业哈尔滨242医院眼科
- 折叠
摘要
Abstract
AlM:To investigate the protective effect and possible mechanisms of lycopene for oxidative damage induced by ultraviolet in cultured human lens epithelial cells ( HLEC) . <br> METHODS: HLEC was subcultured and divided into negative control group, oxidative injury group, lycopene low dose group and lycopene high dose group. Cell viability was assayed by MTT colorimetric. Cell morphological changes were detected by electron microscope. Reactive oxygen species ( ROS) levels were detected with DCFH-DA fluorescent probe. Content of superoxide dismutase ( SOD ) , glutathione peroxidase ( GSH ) and malondialdehyde ( MDA ) in supernatants were detected by spectrophotometer. <br> RESULTS: Lycopene could obviously inhibited UV -induced decline in cell activity, reduce UV-induced ROS generation within HLEC, cause SOD, GSH - Px levels increased and MDA levels decreased. <br> CONCLUSlON:Lycopene plays its strong antioxidant role in increasing the intracellular SOD and GSH - Px content levels and decreasing MDA levels, which provide reliable experimental basis for prevent and treatment of cataracts.关键词
番茄红素/紫外线/晶状体上皮细胞/氧化损伤Key words
lycopene/ultraviolet/human lens epithelial cell/oxidative damage引用本文复制引用
孙敬文,郝静..番茄红素对紫外线诱导人晶状体上皮细胞氧化损伤的保护作用[J].国际眼科杂志,2016,16(5):826-828,3.
