中南医学科学杂志2016，Vol.44Issue(2)：143-146,4.DOI:10.15972/j.cnki.43-1509/r.2016.02.006
干扰miR-21表达抑制胶质瘤U251细胞增殖迁移与侵袭
miR-21 Downregulation Inhibits Cell Proliferation, Migration and Invasion in Glioma
张金灿 1胡丹 1贺礼进 1桂成佳1
作者信息
- 1. 南华大学永州临床学院神经外科，湖南 永州425100
- 折叠
摘要
Abstract
Objective This study was designed to investigate the effect of miR-21 knockdown on cell proliferation,migra-tion and invasion of glioma cell line U251. Methods U251 were transfected with miR-21 inhibitor by Lipofectamine.Meanwhile U251 was transfected with NC inhibitor as negative control.qRT-PCR was used to detect the miR-21 expression in these cells.The experiment was divided in negative control inhibitor group and miR-21 inhibitor group.The effects of miR-21 downregulation on cell proliferation,migration and invasion were evaluated by MTT,wound-healing transwell and invasion assays. Results miR-21 expression was remarkably downregulated in miR-21 inhibitor-transfected cells in concentration-dependent manner, indicating transfection with miR-21 inhibitor can effectively reduce expression level of miR-21 in U251 cells.Transfection of miR-21 inhibitor into U251 cells led to a significant decrease in cell proliferation rate compared with control cells (P<0.01).miR-21 inhibitor re-sults in reduction of cell migration(P<0.01).Moreover,the cell invasion by transwell invasion assay was reduced in miR-21-down-regulated cells relative to control cells. Conclusions miR-21 can promote cell proliferation,migration and invasion of glioma cells.And it maybe plays an important role in tumorigenesis and development of glioma.关键词
胶质瘤/miR-21/细胞增殖/细胞侵袭Key words
glioma/miR-21/cell proliferation/cell invasion分类
医药卫生引用本文复制引用
张金灿,胡丹,贺礼进,桂成佳..干扰miR-21表达抑制胶质瘤U251细胞增殖迁移与侵袭[J].中南医学科学杂志,2016,44(2):143-146,4.
