日用化学工业2016，Vol.46Issue(4)：226-230,242,6.DOI:10.13218/j.cnki.csdc.2016.04.010
灵芝发酵液的成分检测及美白与抗衰老功效评价
Analysis of fermentation liquor of Ganoderma lucidum and evaluation of its whitening and anti-senility efficacy
摘要
Abstract
To develop the application of fermentation liquor of Ganoderma lucidum in the field of cosmetics, Saccharomyces ellipsoideus were used to acquire fermentation liquor Ganoderma lucidum,and the content of total polysaccharides,total polyphenols,total flavones and total polypeptides in the fermentation liquor were determined. The scavenging activity of OH·free radicals and DPPH free radicals as well as the influence on fibroblasts viability were measured to evaluate the possible anti -senility effect of the fermentation liquor. Finally,via in vitro tyrosinase activity test and intracellular tyrosinase activity test on melanin synthesis of mouses as well as melanin inhibition test,the evaluation for whitening effect of the fermentation liquor was conducted. Results showed that the fermentation liquor of Ganoderma lucidum contains 9.910 g/L of total polysaccharides,0.054 g/L of total polyphenols,0.045 g/L of total flavones,and 2.931 g/L of total polypeptides. The fermentation liquor with 25.89% and 42.18% volume fraction of fermentation liquor of Ganoderma lucidum could eliminate 50% OH ·free radical and DPPH free radical. The fermentation liquor with 0.05% -0.2% volume fraction can promote fibroblasts proliferation effectively. The activity of tyrosinase is inhibited significantly by the fermentation liquor of Ganoderma lucidum and the inhibition rate reaches 73.22%. The fermentation liquor of Ganoderma lucidum could inhibit melanin synthesis by decreasing activity of tyrosinase. The fermentation liquor of Ganoderma lucidum exhibits efficacy for anti-senility and whitening.关键词
化妆品添加剂/灵芝/发酵/抗氧化/美白Key words
cosmetic additive/Ganoderma lucidum/fermention/antioxidant/whitening分类
化学化工引用本文复制引用
赵丹,许丹妮,王冬冬,张佳婵,李萌,王昌涛..灵芝发酵液的成分检测及美白与抗衰老功效评价[J].日用化学工业,2016,46(4):226-230,242,6.基金项目
北京工商大学2015年研究生科研能力提升计划资助项目；中国检验检疫科学研究院基本科研业务费装箱资金资助项目（2015JK015）；质检公益性行业科研专项 （）
