日用化学工业2016，Vol.46Issue(4)：238-242,5.DOI:10.13218/j.cnki.csdc.2016.04.012
高效液相色谱法检测防晒类化妆品中的尿刊酸及其乙酯
Determination of urocanic acid and its ethyl ester in sunscreen cosmetics by HPLC
摘要
Abstract
A high performance liquid chromatography (HPLC)was established for the simultaneous determination of urocanic acid and its ethyl ester in sunscreen cosmetics. The result was comfirmed with mass spectrometry. Cosmetic samples were extracted with methanol under assistance of ultrasonic wave and the extract was centrifuged and filtered with a microporous membrane filter. Then the filtrate was transfer for HPLC analysis using an XTerra MS C18 column (4.6 mm × 250 mm,5 μm). The mobile phase was a solution composed of methanol and 5 mmol/L tetrabutylammonium bromide (30∶70)with isocratic elution. The column temperature was set at 35℃. The flow rate was 1 mL/min and the wavelength of detection was at 285 nm. The quantification was carried out by external standard method. The experimental results indicated that the limits of quantification (LOQs)for urocanic acid and its ethyl ester were both 5 mg/kg. The mean recoveries at three spiked levels ranged from 80.1% to 103.8% with the relative standard deviations (RSD)between 1.2% and 6.9%. The suspected positive samples were subjected to further verification by LC -MS/MS. The method is accurate, rapid,sensitive,and can be used for the inspection of sunscreen cosmetics.关键词
防晒化妆品/尿刊酸/尿刊酸乙酯/高效液相色谱Key words
sunscreen cosmetics/urocanic acid/ethyl urocanate/high performance liquid chromatography分类
化学工程引用本文复制引用
郭项雨,马强,孟宪双,李焘,李文雅,白桦..高效液相色谱法检测防晒类化妆品中的尿刊酸及其乙酯[J].日用化学工业,2016,46(4):238-242,5.基金项目
“十二五”国家科技支撑计划资助项目（2013BAK04B03）；质检公益性行业科研专项 （）
