药学研究2016，Vol.35Issue(4)：193-196,211,5.DOI:10.13506/j.cnki.jpr.2016.04.002
半边旗提取物5F诱发结直肠癌细胞生长抑制及细胞凋亡
Growth inhibition and apoptosis in human colorectal cancer cells induced by 5F - extracted from Pteris semipinnata L.
摘要
Abstract
Objective To examine the antiproliferative and proapoptotic effects of 5F(ent - 11α - hydroxy - 15 - oxo- kaur - 16 - en - 19 - oic - acid),a compound isolated from Pteris semipinnata L.(PsL),on HCT - 116 colorectal cancer (CRC)cells. Methods Cytotoxicity was examined using a MTT method. Cell apoptosis was determined through expression of representative apoptosis - related proteins and Annexin V/ PI staining. Reactive oxygen species(ROS)level was meas-ured using standard protocols. Results 5F inhibited the proliferation of HCT - 116 cells in a concentration - and time - de-pendent manner. 5F increased the ratio of Bax/ Bcl - 2 in HCT - 116 cells was identified by western blotting analysis. 5F could enhance both the inhibitory effect of cisplatin on growth of HCT - 116 cells and the level of apoptosis induced by cis-platin in HCT - 116 cells. 5F did not increase ROS,but reduced ROS production induced by cisplatin. Conclusion 5F can induce CRC cell apoptosis and therefore represents therapeutic potential for cancer treatment.关键词
半边旗/结直肠癌/细胞凋亡/活性氧Key words
Pteris semipinnata L./Colorectal cancer/Apoptosis/Reactive oxygen species分类
医药卫生引用本文复制引用
高小胜,吕应年,吴科锋,刘义,符伟玉,陈若泰,李立..半边旗提取物5F诱发结直肠癌细胞生长抑制及细胞凋亡[J].药学研究,2016,35(4):193-196,211,5.基金项目
广东省自然科学基金项目（No. S2012010008163）；湛江市财政资金科技专项竞争性分配项目（No.2013A01008）；广东医学院创新实验项目 （）
