首页|期刊导航|药学研究|HPLC法同时测定小儿解表颗粒中绿原酸、咖啡酸、葛根素、大豆苷的含量

HPLC法同时测定小儿解表颗粒中绿原酸、咖啡酸、葛根素、大豆苷的含量

何海雁张叶刘宏明

药学研究2016，Vol.35Issue(4)：214-216,3.

药学研究2016，Vol.35Issue(4)：214-216,3.DOI:10.13506/j.cnki.jpr.2016.04.008

HPLC法同时测定小儿解表颗粒中绿原酸、咖啡酸、葛根素、大豆苷的含量

Simultaneous determination of chlorogenic acid,caffeic acid,puerarin,daidzin in Xiaoer Jiebiao Granules by HPLC

何海雁 ¹张叶 ²刘宏明³

作者信息

1. 淄博市淄川区食品药品稽查大队，山东淄博 255100
2. 淄博市食品药品检验检测中心，山东淄博 255086
3. 淄博职业学院药学系，山东淄博 255314
折叠

摘要

Abstract

Objective To simultaneous determine the contents of chlorogenic acid,caffeic acid,puerarin,daidzin in Xi-aoer Jiebiao Granules by HPLC. Methods Agilent Zorbax XDB - C18 column(4. 6 mm × 250 mm,5 μm)was adopted with acetonitrile - 0. 1% phosphoric acid as the mobile phases by gradient elution. The column temperature was 40 ℃ . The de-tection wavelength was changed. Results The linear ranges of chlorogenic acid,caffeic acid,puerarin,daidzin were 0. 051 46 ~ 1. 029 2 μg,0. 024 70 ~ 0. 494 0 μg,0. 050 57 ~ 1. 011 4 μg,0. 008 292 ~ 0. 165 8 μg,respectively. The aver-age recoveries were between 98. 77% ~ 99. 98% with RSDs less than 2. 00% . Conclusion The method was accurate,sen-sitive,reproducible,it can be used for the overall assessment of the quality of Xiaoer Jiebiao Granules.

关键词

小儿解表颗粒/高效液相色谱法/绿原酸/咖啡酸/葛根素/大豆苷

Key words

Xiaoer Jiebiao Granules/HPLC/Chlorogenic acid/Caffeic acid/Puerarin/Daidzin

分类

医药卫生

引用本文复制引用

何海雁,张叶,刘宏明..HPLC法同时测定小儿解表颗粒中绿原酸、咖啡酸、葛根素、大豆苷的含量[J].药学研究,2016,35(4):214-216,3.

药学研究

ISSN：2095-5375

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