药学研究2016，Vol.35Issue(4)：217-221,225,6.DOI:10.13506/j.cnki.jpr.2016.04.009
奥沙利铂脂质体的制备及体外释药研究
Preparation of oxaliplatin liposome and in vitro drug - release
摘要
Abstract
Objective To prepare oxaliplatin liposomes with high encapsulation efficiency,and to investigate the in vitro properties of them. Methods Compare oxaliplatin liposomes prepared by several methods,and determine the best pre-scription through the single factor experiment and orthogonal experiment. In the experiment HPLC was used to determine the encapsulation efficiency,and Zetaplus Laser Particle Size Analyzer was used to determine the particle size and zeta potential of liposomes. In addition,both HPLC method and AAS method was used to investigate the in vitro release of the liposomes. Results Compared with thin - film dispersion method and pH gradient method,liposomes prepared by reverse phase evap-oration method presented higher encapsulation efficiency. The best prescription determined by formulation screening was:the ratio of drug to lipid with 1∶7. 5,the ratio of SPC to CH with 2∶1,the ultrasonic power with 195 W and the ultrasonic time with 3 min. In vitro drug release study indicated that the accumulative releasing degree of oxaliplatin liposomes in 24 h was 25. 0% ,determined by HPLC,and 33. 6% ,determined by AAS. Conclusion Liposomes prepared by the reverse phase e-vaporation method were stable enough and assume high encapsulation efficiency and showed the capability of sustained - re-leasing.关键词
奥沙利铂/脂质体/处方筛选/体外释放/高效液相色谱法/原子吸收光谱法Key words
Oxaliplatin/Liposome/Formulation screening/In vitro release/HPLC/AAS分类
医药卫生引用本文复制引用
王健昭,李亚楠,熊晔蓉,沈雁,涂家生,孙春萌..奥沙利铂脂质体的制备及体外释药研究[J].药学研究,2016,35(4):217-221,225,6.基金项目
国家自然科学基金（No.81501579）；江苏省自然科学基金（No. BK20150702）；江苏高校优势学科建设工程资助项目 （）
