山东医药2016，Vol.56Issue(15)：15-18,4.DOI:10.3969/j.issn.1002-266X.2016.15.005
川芎嗪腹腔注射治疗大鼠偏头痛的效果及其作用机制
Effect and mechanism of intraperitoneal injection of tetramethylpyrazine for the treatment of migraine in rats
摘要
Abstract
Objective To explore the effect and mechanism of intraperitoneal injection of tetramethylpyrazine for the treatment of migraine in rats. Methods Thirty Sprague-Dawle ( SD) rats were randomly divided into three groups of 10 in each group:groups A, B and C. Rats in the group A were given intraperitoneal injection of ligustrazine hydrochloride injec-tion before modeling, groups B and C were given intraperitoneal injection of the same volume of normal saline for 7 days. Mi-graine rat model was established by subcutaneously injection of nitroglycerin after 30 min of the last delivery in the groups A and B, and group C with subcutaneous injection of 0. 5 mL normal saline. We observed the frequency of scratching head and climbing cage of each rat. The expression of transient receptor potential cation channel subfamily V member 1 (TRPV1) pro-tein in rat trigeminocervical complex ( TCC) was detected with Western blotting, and the expression of TRPV1 mRNA was de-tected by real-time fluorescence quantificative PCR. Results Compared with group B, the frequency of scratching head and climbing cage in each period was significantly decreased in the groups A and C ( except the frequency of scratching head be-tween groups A and B in the 0-30 min after molding, statistical difference was found, all P<0. 05). Different levels of posi-tive expression of TRPV1 receptors in TCC of each group were observed by immunofluorescence. Compared with groups A and C, the expression intensity was higher in the group B, there was no statistical difference between group A and B. Compared with group B, the protein and mRNA expression of TRPV1 in TCC of group A and C were significantly decreased ( all P<0. 01). Conclusion Intraperitoneal injection of tetramethylpyrazine on migraine rats has a certain therapeutic effect, and the mechanism may be related to the inhibition of TRPV1 expression in trigeminal sensory neurons.关键词
川芎嗪/偏头痛/瞬时受体电位香草酸亚型1/三叉神经颈髓复合体Key words
tetramethylpyrazine/migraine/transient receptor potential cation channel subfamily V member 1/trigem-inal sensory neurons分类
医药卫生引用本文复制引用
白方会,李慧,伍志勇,高媛,聂玲辉,陈宝田..川芎嗪腹腔注射治疗大鼠偏头痛的效果及其作用机制[J].山东医药,2016,56(15):15-18,4.基金项目
国家自然科学基金资助项目(81202687) （81202687）
广东省科技计划项目(2012B061700018) （2012B061700018）
广东省中医药管理局科研课题(20141218)。 （20141218）
