山东医药2016，Vol.56Issue(15)：19-22,4.DOI:10.3969/j.issn.1002-266X.2016.15.006
不同剂量伊桐苷灌胃对大鼠弥散性脑损伤的防治作用及其机制
Preventive and therapeutic effect of different doses of Itoside on rats with diffuse brain injury and its mechanism
摘要
Abstract
Objective To observe the preventive and therapeutic effect of different doses of Itoside on rats with diffuse brain injury ( DBI) and to investigate its mechanism. Methods SD rats were randomly divided into 5 groups: groups A, B, C, D and E. DBI models were prepared in the groups A, B, C and D. Rats in the group E only received scalp incision and skull was exposed. Rats in the groups A, B and C were given Itoside (40, 80 and 160 g/kg) by gavage, respectively, starting from 7 days before modeling, and continuing for 14 days after modeling, once a day. Groups D and E received e-qual volume of saline. We used the sensory and motor function test to evaluate the nerve function′s defect degree after DBI, and observed morphological changes of the cerebral cortex of rats by HE staining. We determined the content of glutathione peroxidase ( GSH-PX) and malondialdehyde ( MDA) through the chemical colorimetric method. Results Compared with group E, neurological score was decreased, pathological changes were significant, the content of MDA in brain tissues was increased, and the activity of GSH-PX was decreased in the group D (all P<0. 01). Compared with group D, neurological score was increased, pathological changes alleviated, the content of MDA in brain tissues was decreased, and the activity of GSH-PX was increased in the groups A, B and C (all P<0. 01). Conclusions Itoside can improve the neural function of rats with DBI and alleviate the pathological damage of brain tissue with the best effect of 160 g/kg Itoside. The mechanism may be related to that Itoside can reduce the formation of oxidation products in the brain tissues and enhance the activity of antioxidant enzymes.关键词
伊桐苷/弥散性脑损伤/丙二醛/谷胱甘肽过氧化物酶Key words
Itoside/traumatic brain injury/malondialdehyde/glutathione peroxidase分类
医药卫生引用本文复制引用
姚瀚勋,唐乐剑,夏学巍,覃超,段震映,王文波..不同剂量伊桐苷灌胃对大鼠弥散性脑损伤的防治作用及其机制[J].山东医药,2016,56(15):19-22,4.基金项目
广西教育厅科研立项项目(10JKT-111) （10JKT-111）
桂林市科学研究与技术开发计划项目(11SKT-66)。 （11SKT-66）
