山东医药2016，Vol.56Issue(15)：30-33,4.DOI:10.3969/j.issn.1002-266X.2016.15.009
结直肠癌组织中Id-1、MMP-9的表达变化及其意义
Expression changes of Id-1 and MMP-9 in colorectal carcinoma tissues and its clinical significance
摘要
Abstract
Objective To observe the expression changes of inhibitor of DNA binding-1 ( Id-1 ) and matrix metallo-proteinase-9 (MMP-9) in the human colorectal carcinoma tissues and to discuss the clinical significance. Methods The expression of Id-1 and MMP-9 in 50 specimens of colorectal carcinoma tissues ( observation group) and 50 specimens of normal colorectal tissues ( control group) was detected by immunohistochemistry, RT-PCR and Western blotting. The cor-relations between their positive expression and the clinicopathological features were analyzed. Results The positive rates of Id-1 in the observation group and the control group were 72% and 24%, the positive rates of MMP-9 were 78% and 22%, respectively, and there were significant differences between these two groups (all P<0. 05). The positive expression of Id-1 and MMP-9 was related with the depth of tumor invasion, TNM stage, lymph node metastasis, vessel invasion and liver metastasis (all P<0. 05). The relative expression of Id-1 mRNA in the observation group and the control group was 0. 96 ± 0. 03 and 0. 20 ± 0. 04, the relative expression of MMP-9 mRNA was 0. 88 ± 0. 04 and 0. 21 ± 0. 03, respectively, and there were significant differences (all P<0. 05). The relative expression of Id-1 protein was 0. 82 ± 0. 04 and 0. 31 ± 0. 02, the relative expression of MMP-9 in the observation group and the control group was 0. 86 ± 0. 05 and 0. 29 ± 0. 03, respectively, and there were significant differences between these two groups (all P<0. 05). Conclusion The expression of Id-1 and MMP-9 was increased in the human colorectal carcinoma tissues, and the expression was related with tumor in-vasion, TNM stage, lymph node metastasis, liver metastasis and vessel invasion.关键词
结肠癌/直肠癌/DNA结合分化抑制蛋白1/基质金属蛋白酶9Key words
colon carcinoma/rectal carcinoma/inhibitor of DNA binding-1/matrix metalloproteinase-9分类
医药卫生引用本文复制引用
武雪亮,王立坤,薛军,杨东东,屈明,郭飞,杨瑞敏,马鹏程,刘博..结直肠癌组织中Id-1、MMP-9的表达变化及其意义[J].山东医药,2016,56(15):30-33,4.基金项目
河北省卫计委医学科学研究重点课题计划(20150058) （20150058）
河北省科技支撑计划项目(152777237) （152777237）
张家口市科技局指令性计划(1311055D)。 （1311055D）
