山东医药2016，Vol.56Issue(16)：1-4,4.DOI:10.3969/j.issn.1002-266X.2016.16.001
慢病毒介导 siRNA 沉默 ERK2对创伤性骨性关节炎大鼠软骨退变的影响及其机制
Effect of siRNA mediated by lentivirus silencing ERK2 on cartilage degeneration in rat models of post traumatic osteoarthritis and its mechanism
摘要
Abstract
Objective To observe the effect of siRNA mediated by lentivirus silencing extracellular signal-regulated kinase 2 (ERK2) on cartilage degeneration in rat models of post-traumatic osteoarthritis and to investigate its mechanism . Methods Thirty light knees of traumatic osteoarthritis model rats were randomly divided into 3 groups with 10 rats in each group, then the right knee joint cavities of theirs were injected with sterile PBS (PBS group), negative ERK2 siRNA lenti-virus solution ( negative control group ) and ERK2 siRNA lentivirus solution ( observation group ) , respectively .After 8 weeks, the rats were sacrificed and the general morphology of the articular cartilage was observed and scored , then the ar-ticular cartilage pathological changes were observed under the optical microscope with HE , toluidine blue and safranin "O"staining, respectively, and the degree of cartilage degeneration was analyzed by Mankin semi-quantitative method.Mean-while, the relative mRNA expression of matrix metalloproteinase 3 (MMP3), MMP13 and Col2a of cartilage tissues was an-alyzed by real-time fluorescence quantitative PCR .Results The articular cartilage surface ulcers and defects in the obser-vation group were significantly lighter than those of the PBS group and negative control group , the extent of smooth articular surface of cartilage, crack area, loss of tissue, matrix staining, the proliferation of chondrocytes and the disorder of ar-rangement in the cartilage tissues of the observation group were lighter than those of the PBS group and negative control group;and the normal morphology and articular cartilage score in the observation group were significantly lower than those in the PBS group and negative control group (all P<0.05), there was no significant difference between the PBS and nega-tive control groups (P>0.05).In addition, the relative mRNA expression levels of MMP3 and MMP13 in the observation group were lower than those in the PBS group and negative control group , while the relative mRNA expression level of Col 2a was higher than that in the PBS group and negative control group (all P<0.01);there was no significant difference in the expression of MMP3, MMP13 and Col2a mRNA between the PBS group and negative control group (all P>0.05).Con-clusion siRNA mediated by lentivirus silencing ERK 2 can significantly reduce the cartilage degeneration of post-traumatic osteoarthritis , whose mechanism may be related with the decreased expression of MMP 3 and MMP13 and increased expres-sion of Col2a.关键词
骨性关节炎/软骨退变/慢病毒/小干扰RNA/细胞外信号调节激酶2/大鼠Key words
osteoarthritis/cartilage degeneration/lentivirus/small interfering RNA/extracellular signal-regulated ki-nase 2/rats分类
医药卫生引用本文复制引用
宁仁德,孔令超,周业金,王祥..慢病毒介导 siRNA 沉默 ERK2对创伤性骨性关节炎大鼠软骨退变的影响及其机制[J].山东医药,2016,56(16):1-4,4.基金项目
国家自然科学基金资助项目（81101380）。 （）
