山东医药2016，Vol.56Issue(16)：8-10,110,4.DOI:10.3969/j.issn.1002-266X.2016.16.003
先天性显性脊椎裂胎鼠宫内干细胞移植新方法的建立
A new method of stem cell transplantation in utero for rats with congenital dominant spine bifida
摘要
Abstract
Objective To establish a novel method of stem cell transplantation in utero for rats with congenital domi -nant spine bifida.Methods Thirty pregnant female rats (16 days, E16), 30 pregnant female rats (17 days, E17) and 26 pregnant female rats (18 days, E18) were selected to establish the animal models of congenital dominant spine bifida . The bone marrow mesenchymal stem cells (MSCs) transfected with green fluorescent protein (GFP) gene were subsequent-ly transplanted into spinal cord of pregnant rats with congenital dominant spine bifida by fetal surgery , microsurgery and mi-croinjection .The E20 pregnant rats were killed and embryo biopsy was performed to count fetal rat survival rate .The spinal cord stem cell transplantation in fetal rat was observed under the fluorescence microscope .Results The survival rate was 94.2%(81/86) for E20 pregnant rats receiving surgery .One hundred ninety-eight embryo rats with stem cell transplanta-tion survived in 258 rats with congenital spinal bifida fetal , whose survival rate was 76.7%(198/258).The survival rates were 72.1%(80/111), 77.3% (78/101) and 87.0% (40/46) in E16, E17, and E18 fetal rates receiving stem cell transplantation .The GFP was observed in embryo spinal cord under fluorescence microscopy .The survival rate of MSCs was 98.5%(195/198).Conclusions We successfully establish an new method of stem cell transplantation in utero for rats with congenital dominant spine bifida and its stem cell survival rate is high .This method can be used as one of the methods in treatment of neural tube defects .关键词
先天性显性脊椎裂/胚胎/显微手术/显微注射/骨髓间充质干细胞/移植/大鼠Key words
congenital dominant spine bifida/embryo/microsurgery/microinjection/bone marrow mesenchymal stem cells/transplantation/rats分类
医药卫生引用本文复制引用
赵桂锋,刘波,苗佳宁,吴迪,李慧,黄天楚,袁正伟..先天性显性脊椎裂胎鼠宫内干细胞移植新方法的建立[J].山东医药,2016,56(16):8-10,110,4.基金项目
国家自然科学基金资助项目（81171072）。 （）
