山东医药2016，Vol.56Issue(16)：11-14,110,5.DOI:10.3969/j.issn.1002-266X.2016.16.004
口腔扁平苔藓患者血清差异蛋白表达检测及意义
Detections of differentially expressed serum proteins in patients with oral lichen planus and the significance
摘要
Abstract
Objective To detect the differentially expressed serum proteins in patients with oral lichen planus ( OLP) and explore their significance .Methods Six OLP patients ( observation group ) and the matched health adults ( control group) were selected and we drew their venous blood .Serum proteins were extracted by phenol extraction .The protein con-centration was determined by Bradford method .Serum proteins were separated by two-dimensional fluorescence difference gel electrophoresis (2-D DIGE), the differential proteins were identified by mess spectrometry and the expression of the proteins was detected by Western blotting in the two groups .Results In average, the number of differential protein spots was (583 ±183), during which, 12 highly reproducible spots were selected for mass spectrometry identification .Of these 12 protein spots, the expression of seven proteins, including serum complement C3, kininogen,α-2-macroglobulin, human antithrombinⅢ, secretory IgA, complement C9 andβ-1-metal binding globulin (SHBG), was decreased in the observation group , the expression of five proteins including haptoglobin , ceruloplasmin , apolipoprotein A-Ⅰ, vitamin D and human factor B was increased in the observation group .Compared with the control group , the expression of serum complement C 3 and human antithrombin Ⅲwas lower, and the expression of apolipoprotein A-Ⅰwas higher in the observation group (all P<0.01). Conclusion There are 12 differential proteins in serum of OLP patients and the expression of serum complement C 3 and hu-man antithrombin Ⅲis lower which shows that the development of OLP may be associated with immune and infection .关键词
口腔扁平苔藓/双向荧光差异凝胶电泳/蛋白质组/补体C3/载脂蛋白AⅠ/人抗凝血酶ⅢKey words
oral lichen planus/two-dimensional fluorescence difference gel electrophoresis/proteomics/serum com-plement C3/apolipoprotein AⅠ/human antithrombin Ⅲ分类
医药卫生引用本文复制引用
刘健,刘铁军,安欣,李昆珊,贾楠,许彦枝..口腔扁平苔藓患者血清差异蛋白表达检测及意义[J].山东医药,2016,56(16):11-14,110,5.基金项目
国家自然科学基金资助项目（81273813）。 （）
