山东医药2016，Vol.56Issue(19)：12-14,3.DOI:10.3969/j.issn.1002-266X.2016.19.004
Notch1基因沉默的人绒毛膜上皮癌细胞增殖及侵袭能力变化
Changes in proliferation and invasive abilities of human chorionic epithelioma cells after silencing Notch1 gene
摘要
Abstract
Objective To observe the changes in the proliferation and invasive abilities of human chorionic epithelio -ma cells after silencing Notch1 gene.Methods We looked up Notch1 sequence (NM 001105721.1) design primers pro-vided by Genbank to synthesize Notch 1 siRNA.Human chorionic epithelioma cells JEG-3 were cultivated and divided into groups A, B and C.The group B was transfected by control siRNA , group C was transfected by Notch1 siRNA and group A without any transfection.12, 24, 36,48, 60 hours after transfection, the cell proliferation ability was determined by MTT in each group , and the invasion ability was detected by Transwell invasive experiment .The expression of MMP-2 and MMP-9 protein was detected by Western blotting 72 h after transfection .Results The cell proliferation ability of group C at 36 h, 48 h and 60 h after transfection was lower than those of groups A and B (all P<0.05).The number of cells penetrating the membrane in the groups A , B and C were 109 ±6 , 118 ±7 and 59 ±4;significant difference was found between group C and groups A and B (all P<0.05).The relative expression of MMP-2 of groups A, B and C was 0.91 ±0.08, 0.87 ± 0.01 and 0.29 ±0.05, respectively.The relative expression of MMP-9 of groups A, B and C was 0.85 ±0.04, 0.79 ± 0.03 and 0.35 ±0.05, respectively.The expression of MMP-2 and MMP-9 in the group C was lower than that of groups A and B (all P<0.05).Conclusion The cell proliferation of human chorionic epithelioma is inhibited and the invasive a-bility is decreased after silencing Notch 1 gene.关键词
绒毛膜上皮癌/Notch1基因/RNA干扰技术Key words
chorionic epithelioma/Notch1 gene/RNA interference分类
医药卫生引用本文复制引用
刘绍晨,许倩,李健会..Notch1基因沉默的人绒毛膜上皮癌细胞增殖及侵袭能力变化[J].山东医药,2016,56(19):12-14,3.基金项目
河北省高等学校科学研究计划项目（QN2014011）；河北省病理学与病理生理学重点学科；河北省人口和计划生育委员会科技研究计划项目（ZL20140231）。 （）
