山东医药2016，Vol.56Issue(19)：19-21,3.DOI:10.3969/j.issn.1002-266X.2016.19.006
肝癌患者外周血中循环肿瘤细胞的检测及其应用
Detection and application of circulating tumor cells in peripheral blood of patients with liver cancer
摘要
Abstract
Objective To observe the circulating tumor cells (CTC) in the peripheral blood of patients with liver cancer and to investigate the application of CTC detection in the early diagnosis and monitoring treatment of liver cancer .Methods Twenty-seven patients with primary liver cancer were selected as the liver cancer group and 30 healthy person were selected as the control group.The peripheral blood was collected, and we enriched the CTC by subtraction enrichment technology and identified them by fluorescence in situ hybridization (FISH) technique.The serum tumor markers AFP, carcino-embryonic antigen (CEA) and CA199 of the two groups were detected by electrochemiluminescence method .The sensitivity, specificity and youden index of CTC, AFP, CEA and CA199 were comprehensively compared, and the relationship between CTC and therapeutic effect of targe-ted therapy was analyzed.Results CTC was detected in 22 cases of the liver cancer group, while in the control group the num-ber was 0 (P<0.05).C subset of tumor cells was detected in 22 cases of the liver cancer group while there was only 1 case of A subset and 1 case of B subset.Polyploidy tumor cells were all detected in patients with positive CTC, and the triploid took the largest proportion (59.8%).In the liver cancer group, 17 cases were positive AFP, 10 cases were positive CEA and 11 cases were positive CA199, while the numbers in the control group were respectively 2, 1 and 0.The youden index of CTC detection was better than the other three tumor markers in detection and diagnosis (P<0.05).Ten patients in the liver cancer group insis-ted taking target drugs, the number of CTC was stable in 8 patients, the RECIST was SD.The number of CTC declined (<5/7.5 mL) in 2 patients with high TCT number (>15/7.5 mL) after taking one-week Sorafenib, while the RECIST was also SD. Conclusions CTC in the peripheral blood of patients with liver cancer could be detected effectively by subtraction enrichment technique.Compared with common tumor markers, the CTC detection shows more advantages, and it could be used to assess the effect of targeted therapy in patients with liver cancer.关键词
循环肿瘤细胞/肝肿瘤/肝癌/肿瘤标志物Key words
circulating tumor cells/liver neoplasms/liver cancer/tumor biomakers分类
医药卫生引用本文复制引用
马清,贾茹,张春艳,金欣,左向华,林平,徐建明,陈水平,陈建魁..肝癌患者外周血中循环肿瘤细胞的检测及其应用[J].山东医药,2016,56(19):19-21,3.基金项目
首都临床特色应用研究项目（Z121107005112001）；国家高技术研究发展计划项目（2011AA02A111）。 （）
