山东医药2016，Vol.56Issue(19)：22-24,3.DOI:10.3969/j.issn.1002-266X.2016.19.007
体外培养的人恶性黑色素瘤球体细胞干细胞特性观察
Stem cell characteristics of human malignant melanoma sphere cells cultured in vitro
摘要
Abstract
Objective To observe the stem cell properties of human malignant melanoma cells cultured in serum-free me-dium.Methods Human malignant melanoma A375 cell line was cultured in serum-free medium, and the melanoma sphere cells (sphere group) and common adherent cells (adherent group) were obtained.Cell migration was detected using Transwell cham-ber.CD20 and CD133 were detected by immunofluorescence.Sixteen BALB/c mice were divided into the experimental group and control group (8 rats in each group), and the sphere cells and the adherent cells of 1 mL (104 ) were injected into the mouse shoulder blade by subcutaneous injection in the sphere and the adherent groups, respectively.The tumor cells were observed and compared between the two groups.Six weeks later, the mice were killed to obtain the tumor tissues.Results A375 cells were seeded in serum-free medium, visible small round ball suspension cells formed and gradually becomes larger , the ball inside the cells were closely connected with good refraction.The cell migration ability and the expression intensity of CD20 and CD133 in the sphere group were higher than those in the adherent group .After 6 weeks of injection, the mice in the experimental group had a large tumor, and no tumor growth was found in the control group.The tumor tissues of the experimental group were stained with HE, and the malignant melanoma was pathologically diagnosed.Conclusions Human malignant melanoma cells cultured in serum-free medium have the characteristics of stem cells with high migration ability and tumorigenesis .High expression of im-mune markers CD20, CD133 is found and malignant melanoma exists in the tumor stem cells.关键词
黑色素瘤/肿瘤干细胞/无血清培养基Key words
melanoma/tumor stem cells/serum-free medium分类
医药卫生引用本文复制引用
文辉才,眭云鹏,简雪平,廖怀伟,马丽,徐桂珍,刘燕平,万珺..体外培养的人恶性黑色素瘤球体细胞干细胞特性观察[J].山东医药,2016,56(19):22-24,3.基金项目
江西省科技支撑计划项目（2010BSA14600）。 （）
