色谱2016，Vol.34Issue(5)：512-519,8.DOI:10.3724/SP.J.1123.2015.12020
固相萃取结合气相色谱-串联质谱法测定烟草制品中23种酯类香料
Analysis of 23 esters of flavor additive in tobacco products by gas chromatography-tandem mass spectrometry coupled with solid-phase extraction
摘要
Abstract
An analytical method for the simultaneous determination of 23 esters of flavor addi-tive in tobacco products was established based on optimized solid-phase extraction( SPE)with gas chromatography-tandem mass spectrometry ( GC-MS/MS ). The samples were extracted with ethyl acetate,cleaned up by an amino SPE column. After conditioning step,the extracts were eluted by 4 mL ethyl acetate,and then were determined by GC-MS/MS in multi-reaction monitoring( MRM)mode. The results showed that all the 23 ester flavor additives had good lin-earity in the range of 20. 0-500. 0 μg/L,with the correlation coefficients(r2)better than 0. 996. The limits of detection( LODs)and limits of quantitation( LOQs)of these additives were in the ranges of 1. 9-13. 6 μg/kg and 6. 3-45. 3 μg/kg,respectively. The average recoveries of the 23 analysts were in the range of 62. 1%-91. 4% at the spiked levels of 1,1. 5,2 times LOQ with rel-ative standard deviations( RSDs)typically lower than 7%. The accurate,reliable and sensitive method can be applied to the determination of the 23 esters of flavor additive in tobacco prod-ucts for rapid screening and quantitative analysis. The method also offered an opportunity to detect more ester additives that may be added in tobacco products.关键词
固相萃取/气相色谱-串联质谱/酯类香料/烟草制品Key words
solid phase extraction( SPE )/gas chromatography-tandem mass spectrometry ( GC-MS/MS)/ester flavor additives/tobacco products分类
化学化工引用本文复制引用
郑阳,许秀丽,纪顺利,袁飞,黄志强,杨丙成,张峰..固相萃取结合气相色谱-串联质谱法测定烟草制品中23种酯类香料[J].色谱,2016,34(5):512-519,8.基金项目
质检公益性行业科研专项（201410088，201310134）；中国检验检疫科学研究院基本科研业务费项目（2016JK009，2015JK008）；国家质检总局科研计划项目（2014IK084）；国家重大仪器设备开发专项（2012YQ14000806）．@@@@Public Welfare Fund of Inspection and Quarantine of China（ Nos．201410088，201310134）；Fund of Chinese Academy of Inspection and Quarantine（ Nos．2016JK009，2015JK008）；Science and Technology Project of Administration of Quality Supervision，Inspection and Quarantine of China（ No．2014IK084）；National Key Scientific Instrument and Equipment Development Projects of China（ No．2012YQ14000806）． （）
