实用肝脏病杂志2016，Vol.19Issue(3)：270-274,5.DOI:10.3969/j.issn.1672-5069.2016.03.005
甘丙肽体外抑制脂多糖诱导的小鼠RAW264.7巨噬细胞促炎功能研究
Inhibitory effect of galanin on proinflammatory response induced by lipopolysaccharides in a mouse RAW264.7 macrophage cell line in vitro
摘要
Abstract
Objective To investigate the effect of galanin on mouse RAW264.7 macrophage cell line. Methods RAW264.7 mouse macrophage cells were cultured in DMEM (100 U/ml of penicillin and 100g/ml of streptomycin) containing 10% FBS at 37℃,in a humidified 5% CO2 atmosphere. Cells were divided into three groups,namely the control group (vehicle only),lipopolysaccharides (LPS) group(LPS only) and galanin treatment group (LPS plus galanin). Cells were serum-starved for 4 h followed by LPS(1μg/ml) or vehicle incubation for 2 h;Cell were then incubated with or without galanin (1000nmol/L) for 24 h. Cell morphology was observed under the microscope;mRNA levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and interleukin-1β(IL-1β) were measured by real-time RT-PCR and Western blotting analysis were performed to determine the inducible nitric oxide synthase (iNOS) and arginase 1 (Arg1)protein levels. Results The mRNA levels of IL-6,IL-1β and TNF-α in LPS group was increased by (7083 ± 432.5),(3106 ± 104.3) and (108.0±47.58) folds of that in the control group,respectively(P<0.05);and the mRNA levels of IL-6,IL-1β and TNF-α in cells treated with galanin decreased by(1.2±0.2),(0.3±0.08) and(19±1.3) folds,respectively,as compared with LPS group(P<0.05);Relative level of iNOS protein in LPS group increased by (19.5±1.964) folds compared with that of the control group,and relative level of Arg1 protein in LPS group was decreased by (1.6±0.3) folds compared with that of the control group;After galanin treatment,iNOS protein level decreased by (1.7±0.32) folds and Arg1 protein increased by (2.31±0.12) folds lower or greater than that treated with LPS alone (P<0.05). Conclusions Galanin exhibits a significant inhibitory effect on proinflammatory response induced by LPS in mouse Raw264.7macrophage in vitro.关键词
RAW264.7巨噬细胞系/甘丙肽/细胞因子/精氨酸酶1/体外Key words
RAW264.7 macrophage cell line/Galanin/Cytokines/Arginase 1/In vitro引用本文复制引用
何玲楠,李兵航,周达,梁灿灿,丁永年,陈源文,范建高..甘丙肽体外抑制脂多糖诱导的小鼠RAW264.7巨噬细胞促炎功能研究[J].实用肝脏病杂志,2016,19(3):270-274,5.基金项目
新疆维吾尔自治区高校科研计划重点项目（编号XJEDU2012I001）；国家自然科学基金项目（编号81170410/81260081）；上海市卫生系统优秀青年人才培养计划项目 （）
